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在猪冠状动脉平滑肌微粒体部分中证明一种可能与钙离子转运相关的（钙离子 + 镁离子）-ATP酶活性。

Demonstration of a (Ca2+ + Mg2+)-ATPase activity probably related to Ca2+ transport in the microsomal fraction of porcine coronary artery smooth muscle.

作者信息

Wuytack F, Casteels R

出版信息

Biochim Biophys Acta. 1980 Jan 25;595(2):257-63. doi: 10.1016/0005-2736(80)90088-7.

DOI:10.1016/0005-2736(80)90088-7
PMID:6444357
Abstract

A (Ca2+ + Mg2+)-ATPase activity is demonstrated in the microsomal fraction of porcine coronary artery. The characteristics of the ATPase activity are compared with those of the Ca2+ transport, both measured in similar solutions. It is concluded that the (Ca2+ + Mg2+)-ATPase is related to the Ca2+ transport because: 1. Both transport and ATPase have similar low Km values for Ca2+ as well as comparable Hill coefficients. The Km values are respectively 0.34 +/- 0.03 microM [4] and 1.17 +/- 0.15 microM [6]. The Hill coefficients are n = 1.69 +/- 0.09 [4] and n = 1.23 +/- 0.17 [6]. 2. Ionophores A23187 and X537A stimulate (Ca2+ + Mg2+)-ATPase activity while they inhibit net Ca2+ accumulation by increasing the Ca2+ permeability of the membranes. 3. The V values for Ca2+ accumulation and for (Ca2+ + Mg2+)-ATPase are comparable.

摘要

在猪冠状动脉微粒体部分证实存在（Ca2+ + Mg2+）-ATP酶活性。将该ATP酶活性的特征与在相似溶液中测得的Ca2+转运的特征进行了比较。得出结论：（Ca2+ + Mg2+）-ATP酶与Ca2+转运相关，原因如下：1. 转运和ATP酶对Ca2+均具有相似的低Km值以及相当的希尔系数。Km值分别为0.34±0.03微摩尔[4]和1.17±0.15微摩尔[6]。希尔系数分别为n = 1.69±0.09[4]和n = 1.23±0.17[6]。2. 离子载体A23187和X537A刺激（Ca2+ + Mg2+）-ATP酶活性，同时它们通过增加膜对Ca2+的通透性来抑制净Ca2+积累。3. Ca2+积累和（Ca2+ + Mg2+）-ATP酶的V值相当。

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