Wide M
Teratology. 1978 Apr;17(2):165-9. doi: 10.1002/tera.1420170209.
In vitro culture of mouse blastocysts over the implantation period was used (1) to find out whether embryos recovered from uteri of mice given an injection of lead, which has been shown in a previous study to inhibit implantation in vivo, were capable of attachment and outgrowth when transferred to a lead free culture medium; (2) to study the effect of different amounts of inorganic lead in the culture medium on blastocyst viability and ability to attach and grow out. The levels of lead used in the medium were comparable to those found in uterine tissue of animals given an implantation inhibiting dose of lead. It was found that the preimplantation blastocyst was unaffected by the administration of lead to the mother as regards the ability to attach and grow out. It was also shown that normal blastocysts were adversely affected by the presence of lead in the culture medium. Addition of increasing amounts of inorganic lead (5, 10 and 20 mumol/l) caused a corresponding decrease in the ability of the blastocysts to attach and grow out, the highest concentration also causing abnormal appearance of the embryos within 48 hours of culture.
采用小鼠胚泡在着床期进行体外培养的方法，目的如下：（1）研究从经注射铅的小鼠子宫中回收的胚胎，在转移至无铅培养基后是否能够附着并生长。先前的一项研究表明，铅在体内可抑制着床；（2）研究培养基中不同含量的无机铅对胚泡活力以及附着和生长能力的影响。培养基中使用的铅含量与给予抑制着床剂量铅的动物子宫组织中的铅含量相当。结果发现，就附着和生长能力而言，着床前胚泡不受母体给予铅的影响。研究还表明，培养基中铅的存在会对正常胚泡产生不利影响。添加越来越多的无机铅（5、10和20μmol/L）会导致胚泡附着和生长能力相应下降，最高浓度还会在培养48小时内导致胚胎外观异常。
