Vazeille M C, Chevalier M
C R Seances Soc Biol Fil. 1980;173(6):1064-9.
Minced whole testes from immature pigs were treated with collagenase (0.4 mg/ml) then with trypsin (2.5 mg/ml). The resulting suspension of cells was divided into sterile bottles containing medium 199 supplemented with 5 p. 100 calf serum. Bottles were maintained at 32 degrees C. After about 24 hours medium was changed 3 times to eliminate free cells. Within 3 days preparations consisting of 81.5% Sertoli cells, 0.5% Leydig cells and 18% peritubular cells were obtained.
取未成熟猪的整个睾丸切碎，先用胶原酶（0.4毫克/毫升）处理，再用胰蛋白酶（2.5毫克/毫升）处理。将所得细胞悬液分装到含有添加了5%小牛血清的199培养基的无菌瓶中。将瓶子置于32摄氏度下培养。约24小时后更换培养基3次以去除游离细胞。在3天内获得了由81.5%的支持细胞、0.5%的间质细胞和18%的睾丸周细胞组成的细胞制剂。
