Hidaka H, Omura S, Oda T
Acta Med Okayama. 1980 Feb;34(1):1-10. doi: 10.18926/AMO/30565.
Several DNA-histone complexes were reconstituted in the presence and absence of urea. The fiber size of DNA-histone H1 complex was about 20 A in width with knobs 100 to 250 A in diameter interspersed at an average interval of about 1,100 A. H1-was associated with DNA segments corresponding to a DNA size of fewer than 100 base pairs. DNA-histones H2A, H2B, H3 and H4 complex consisted of globular subunits 100 to 150 A in diameter alternating with thin strands, like beads on a string. DNA-whole histones complex was 200 to 250 A in width and had a condensed configuration. The nuclease digestion pattern of the complexes containing histones H2A, H2B, H3 and H4 was regular, similar to that of chromatin, and was disrupted by urea. The complex containing H1 was inactive for in vitro RNA synthesis by escherichia coli RNA polymerase, whereas the other complexes were active. The complexes reconstituted in the absence of urea had template activities slightly less than in the presence of urea.
在有尿素和无尿素的情况下重构了几种DNA-组蛋白复合物。DNA-组蛋白H1复合物的纤维宽度约为20埃，直径100至250埃的节段以约1100埃的平均间隔散布。H1与对应于少于100个碱基对DNA大小的DNA片段相关联。DNA-组蛋白H2A、H2B、H3和H4复合物由直径100至150埃的球状亚基与细链交替组成，就像串珠一样。DNA-全组蛋白复合物宽度为200至250埃，具有浓缩结构。含有组蛋白H2A、H2B、H3和H4的复合物的核酸酶消化模式是规则的，类似于染色质，并且被尿素破坏。含有H1的复合物对大肠杆菌RNA聚合酶的体外RNA合成无活性，而其他复合物有活性。在无尿素情况下重构的复合物的模板活性略低于有尿素时的活性。
