Graham L M, Vinter D W, Ford J W, Kahn R H, Burkel W E, Stanley J C
Arch Surg. 1980 Aug;115(8):929-33. doi: 10.1001/archsurg.1980.01380080025005.
Thirteen adult dogs underwent thoracoabdominal bypass operations with 6-mm, double-velour Dacron grafts 25 to 30 cm long. Experimental grafts were seeded with cultured autologous endothelial cells (n = 7). Unseeded grafts served as controls (n = 6). Endothelial cells were harvested from external jugular vein segments using 0.1% trypsin and 0.5% collagenase solutions. Grafts were studied at weeks 2 and 4. Endothelial cell coverage of experimental graft surfaces after two weeks was 60% to 70%, and approximately 80% after four weeks. Immunofluorescence using factor VIII-related antigen confirmed the graft's inner surface to be endothelium. Endothelial cell coverage in control grafts occurred as pannus ingrowth, and never exceeded more than 10% of the conduit surface. Generation of an early endothelial surface in prosthetic grafts is possible in a canine model using cultured autologous cells.
13只成年犬接受了胸腹分流手术，使用的是长度为25至30厘米的6毫米双绒面涤纶移植物。将培养的自体内皮细胞接种到实验性移植物上（n = 7）。未接种细胞的移植物作为对照（n = 6）。使用0.1%胰蛋白酶和0.5%胶原酶溶液从颈外静脉段采集内皮细胞。在第2周和第4周对移植物进行研究。两周后实验性移植物表面的内皮细胞覆盖率为60%至70%，四周后约为80%。使用因子VIII相关抗原的免疫荧光证实移植物的内表面为内皮。对照移植物中的内皮细胞覆盖是由于血管翳向内生长，且从未超过导管表面的10%。在犬模型中，使用培养的自体细胞在人工移植物中生成早期内皮表面是可行的。
