Haeberli A, Straub P W
J Lab Clin Med. 1980 Aug;96(2):258-66.
In order to measure quantitatively fibrin and fibrinogen in biological material such as thrombi, exudates, or tissues, the following method was developed and tested on model thrombi of different composition. Any fibrin and fibrinogen present is proteolytically degraded by endogenous and/or exogenous plasmin to their common late, soluble fragments D and E. Fragment E is determined by electroimmunoassay using a specific antiserum. From the amounts of fragment E the original fibrin(fibrinogen) antigen and thus of fibrin plus fibrinogen can be calculated on the basis of a molecular weight ratio of 340,000 for fibrinogen to 48,500 for fragment E. Purified fibrinogen degraded to fragments by plasmin resulted in a yield of 89.9% +/- 1.5 of the expected amount of fragment E. Fibrin under identical conditions resulted in a yield of 84.0% +/- 2.4, whereas crosslinked fibrin gave yields of 78.2% +/- 9.2 only after prolonged incubation. Control studies demonstrated that the presence of plasma proteins did not change the yield of fragment E. Measurement of the fibrin(fibrinogen) content of thrombotic material contained in surgically removed human aortic aneurysms demonstrated the validity and applicability of this method to biological material.
为了定量测量生物材料（如血栓、渗出液或组织）中的纤维蛋白和纤维蛋白原，开发了以下方法，并在不同成分的模型血栓上进行了测试。存在的任何纤维蛋白和纤维蛋白原都会被内源性和/或外源性纤溶酶蛋白水解降解为它们常见的晚期可溶性片段D和E。使用特异性抗血清通过电免疫测定法测定片段E。根据纤维蛋白原与片段E的分子量比为340,000比48,500，从片段E的量可以计算出原始纤维蛋白（纤维蛋白原）抗原，进而计算出纤维蛋白加纤维蛋白原的量。纤溶酶将纯化的纤维蛋白原降解为片段，片段E的产量为预期量的89.9%±1.5。在相同条件下，纤维蛋白的产量为84.0%±2.4，而交联纤维蛋白仅在长时间孵育后产量为78.2%±9.2。对照研究表明，血浆蛋白的存在不会改变片段E的产量。对手术切除的人类主动脉瘤中所含血栓物质的纤维蛋白（纤维蛋白原）含量的测量证明了该方法对生物材料的有效性和适用性。
