Jerez C, Weissbach H
J Biol Chem. 1980 Sep 25;255(18):8706-10.
The methylation of newly synthesized ribosomal protein L11 has been obtained in an in vitro system using lambda rifd 18 DNA as template and S-adenosyl[3H]methionine as methyl donor. About four methyl groups are incorporated per mol of L11 synthesized and the bulk of the methyl groups are present in the protein as trimethyl-lysine. lambda rifd 18 DNA also contains the genes for rRNA and newly synthesized 16 S and 23 S RNA are also methylated in this in vitro system.
在一个体外系统中，以λrifd 18 DNA为模板，S-腺苷[3H]甲硫氨酸为甲基供体，实现了新合成的核糖体蛋白L11的甲基化。每合成1摩尔L11约有4个甲基被掺入，且大部分甲基以三甲基赖氨酸的形式存在于蛋白质中。λrifd 18 DNA还包含rRNA的基因，在这个体外系统中，新合成的16 S和23 S RNA也会被甲基化。
