Yamada G, Takahashi T, Mizuno M, Sakamoto Y, Kaga K, Kobayashi T, Nagashima H
Acta Med Okayama. 1980 Jun;34(3):175-87. doi: 10.18926/AMO/30558.
A recently modified method using peroxidase labeled antibodies for light and electron microscopic demonstration of hepatitis B virus (HBV) was applied to the evaluation of hepatitis B surface antigen (HBsAg) on the surface of liver cells in biopsy specimens from 24HBsAg chronic carriers. Membranous distribution of HBsAg was demonstrated in diffuse or scattered hepatocytes in all 4 asymptomatic carriers and in 3 of the 20 patients with HBsAg-positive chronic active hepatitis or liver cirrhosis. In these patients with membranous expression of HBsAg, hepatitis B e antigen, Dane particles and DNA polymerase were often detected in sera, and large amounts of hepatitis B core antigen appeared in the liver. These results suggest that membrane-bound HBsAg may be expressed by the HBV genome. The ultrastructural study of liver cells showing membranous expression disclosed dense deposits of reaction product indicative of HBsAg on the cell membrane and/or on assembled particles within the extracellular space. In some hepatocytes showing both diffuse cytoplasmic and membranous expression of HBsAg, HBsAg-positive membrane of cisternae open to the intercellular space was connected with the liver cell membrane. These findings supported the conjecture that HBV associated antigens are integrated into the liver cell membrane.
一种最近改良的使用过氧化物酶标记抗体的方法，用于光镜和电镜下乙型肝炎病毒（HBV）的显示，被应用于对24例HBsAg慢性携带者活检标本中肝细胞表面乙型肝炎表面抗原（HBsAg）的评估。在所有4例无症状携带者以及20例HBsAg阳性慢性活动性肝炎或肝硬化患者中的3例患者的散在或弥漫性肝细胞中，均显示出HBsAg的膜状分布。在这些有HBsAg膜状表达的患者中，血清中常检测到乙型肝炎e抗原、丹氏颗粒和DNA聚合酶，并且肝脏中出现大量乙型肝炎核心抗原。这些结果提示，与膜结合的HBsAg可能由HBV基因组表达。对显示膜状表达的肝细胞进行超微结构研究发现，在细胞膜和/或细胞外空间内组装颗粒上有密集的反应产物沉积物，提示为HBsAg。在一些同时显示HBsAg弥漫性胞质和膜状表达的肝细胞中，通向细胞间隙的池的HBsAg阳性膜与肝细胞膜相连。这些发现支持了HBV相关抗原整合到肝细胞膜中的推测。
