Herrmann H J
Gegenbaurs Morphol Jahrb. 1980;126(2):269-72.
The choice of a procedure for measurements of blood vessels (b.v.) should depend on the type of b.v. investigated: For vessels defined anatomically and in respect to their function of organ supply the method of SUWA et al. (1961) is the most adequate one, provided that b.v. dilatations developing during lifetime and persisting after death as a result of special methods of fixation or shock-freezing or being generated by postmortal perfusion can be excluded. Changes in wall thickness of defined b.v. sectioned transversally can also be determined by measurement of the wall area and/or the maximal chord length. At undefined vessels procedures which measure the wall-to-lumen ratio are useless, because lumen changes are not quantifiable. Thus lumen changes can mask of mimick changes in wall thickness. The especially interesting b.v. either with a pathologically changed or without an elastic membrane are not measurable. The unsable method to detect changes in wall thickness at undefined b.v. is founded on the determination of the vessel wall area and/or the number of all the b.v. classified according to their diameter of a certain organ region. But the investigation of the smallest precapillary vessels presupposes their visualization by a special modification of the ATPase reaction.
对于根据解剖结构及其器官供血功能定义的血管，若能排除因特殊固定方法、速冻或死后灌注产生的、在生前出现并在死后持续存在的血管扩张，SUWA等人（1961年）的方法是最合适的。对于横向切片的特定血管，其壁厚变化也可通过测量壁面积和/或最大弦长来确定。对于未明确的血管，测量壁腔比的方法毫无用处，因为腔的变化无法量化。因此，腔的变化可能掩盖或模拟壁厚的变化。特别值得关注的是，要么有病理改变要么没有弹性膜的血管无法测量。通过确定血管壁面积和/或根据特定器官区域直径分类的所有血管数量来检测未明确血管壁厚变化的方法不可行。但是，对最小的毛细血管前血管进行研究需要通过对ATP酶反应进行特殊改良来使其可视化。
