Kwiatkowska D, Dawiskiba J, Kwiatkowska J
Neoplasma. 1980;27(4):415-22.
An increase in lactate production, and in activities of phosphohexoisomerase, phosphofructokinase and pyruvate kinase was found in erythrocytes of patients with advanced cancer disease. Phosphofructokinase isolated from patients' erythrocytes showed enhanced affinity for substrate and coenzyme, diminished thermal stability and changed dependence of the activity on pH. Allosteric properties of the enzyme were modified. A decrease in glucose-6-phosphate dehydrogenase activity was observed in hemolysate. The partially purified enzyme showed decreased affinity for glucose-6-phosphate, and markedly reduced stability at 45 degrees C and in the acid and alkaline pH range. Changes in kinetic and molecular properties of the two key enzymes of glucose metabolism may contribute to hemolysis observed in many cancer patients. Incubation in vitro of normal human erythrocytes with blood sera of patients resulted in increasing of phosphofructokinase, and decreasing of glucose-6-phosphate dehydrogenase activity, indicating that an acquired enzymopathy may be present in cancer.
在晚期癌症患者的红细胞中，发现乳酸生成增加，磷酸己糖异构酶、磷酸果糖激酶和丙酮酸激酶的活性增强。从患者红细胞中分离出的磷酸果糖激酶对底物和辅酶的亲和力增强，热稳定性降低，活性对pH的依赖性改变。该酶的变构性质发生了改变。在溶血产物中观察到葡萄糖-6-磷酸脱氢酶活性降低。部分纯化的该酶对葡萄糖-6-磷酸的亲和力降低，在45℃以及酸性和碱性pH范围内稳定性明显降低。葡萄糖代谢的两种关键酶的动力学和分子性质变化可能导致许多癌症患者出现溶血现象。正常人红细胞与患者血清在体外孵育导致磷酸果糖激酶活性增加，葡萄糖-6-磷酸脱氢酶活性降低，这表明癌症患者可能存在获得性酶病。
