Brahmacupta P, Melnykovych G
J Cell Physiol. 1980 Nov;105(2):227-33. doi: 10.1002/jcp.1041050206.
Parallel changes in the enzyme activities of CA2+ATPase and alkaline phosphatase were observed in HeLa cells. Both enzymes were inhibited to a similar degree by L-phenylalanine, L-tryptophan, and L-leucine, while being relatively resistant to L-homoarginine. Exposure to heat (56 degrees C, 60 degrees C, and 65 degrees C) resulted in a loss of both enzyme activities. Both alkaline phosphatase and Ca2+ ATPase, when treated with EGTA, required Ca2+ for the restoration of activity. Cells grown in the presence of agents that affect alkaline phosphatase (dexamethasone, butyric acid, and hyperosmolar NaCl) showed similar changes in the activities of both enzymes.
在HeLa细胞中观察到Ca²⁺ATP酶和碱性磷酸酶的酶活性发生平行变化。这两种酶都受到L-苯丙氨酸、L-色氨酸和L-亮氨酸相似程度的抑制，而对L-高精氨酸相对耐受。暴露于热（56℃、60℃和65℃）导致两种酶活性丧失。当用乙二醇双四乙酸（EGTA）处理时，碱性磷酸酶和Ca²⁺ATP酶恢复活性都需要Ca²⁺。在存在影响碱性磷酸酶的试剂（地塞米松、丁酸和高渗氯化钠）的情况下生长的细胞，两种酶的活性显示出相似的变化。
