Hannouche N, Samperez S, Jouan P
C R Seances Soc Biol Fil. 1980;174(6):957-62.
The binding of gonadotropin-releasing hormone (Gn-RH) to membranes of male rats pituitary cells was studied. It appeared that Gn-RH was bound to 3 classes of sites and that the concentration in high affinity binding sites was very low. Moreover there was negatively cooperative interaction among these high affinity binding sites leading to a rapid decrease in binding affinity and consequently unmeasurable displacement of labeled hormone with large amounts of radio-inert ligand. These facts are inconsistent with the possible estimation of Gn-RH by radioreceptor assay.
研究了促性腺激素释放激素（Gn-RH）与雄性大鼠垂体细胞膜的结合情况。结果显示，Gn-RH与3类位点结合，且高亲和力结合位点的浓度非常低。此外，这些高亲和力结合位点之间存在负协同相互作用，导致结合亲和力迅速降低，因此用大量放射性惰性配体无法检测到标记激素的置换。这些事实与通过放射受体分析法对Gn-RH进行可能的估计不一致。
