Benestad H B, Rytömaa T, Svensson T
Exp Hematol. 1980 Sep;8(8):961-70.
Inhibition of cell proliferation by leucocyte extracts containing the granulocytic chalone has been monitored in various ways. Target cells were rat bone marrow cells and chloroleukaemia cells cultured on cover glasses, as well as mouse marrow cells cultured in vivo and in vitro in diffusion chambers. Provided that the technique is optimized, the in vitro rat cell assays yielded reliable results with different types of radioactive DNA precursors. With the diffusion chamber techniques cells synthesizing DNA were killed specifically by hydroxyurea, rather than labelling them with e.g. 3H-thymidine. Inhibition of proliferation then showed up as a decreased cell killing, as compared with the controls. Inhibition of proliferation of both progenitor and transit cells could be demonstrated in this way, without interference by added thymidine.
含有粒细胞抑素的白细胞提取物对细胞增殖的抑制作用已通过多种方式进行监测。靶细胞是培养在盖玻片上的大鼠骨髓细胞和氯白血病细胞，以及在扩散小室中体内和体外培养的小鼠骨髓细胞。只要技术得到优化，体外大鼠细胞试验使用不同类型的放射性DNA前体就能产生可靠的结果。使用扩散小室技术时，合成DNA的细胞会被羟基脲特异性杀死，而不是用例如3H-胸腺嘧啶核苷对它们进行标记。与对照组相比，增殖抑制表现为细胞杀伤减少。通过这种方式可以证明祖细胞和过渡细胞的增殖均受到抑制，且不受添加胸腺嘧啶的干扰。
