Chant S, Katz A, Silverman M
J Clin Lab Immunol. 1980 Nov;4(3):133-40.
The pathogenicity of highly purified brush border membrane (BBM) antigens in Heymann Nephritis was compared to that of a crude renal tubular Fx1A preparation and was found to be equally pathogenic. Both antigen preparations induced formation of autologous antisera. Double immunodiffusion studies demonstrated a reaction of identity between anti-BBM and anti-Fx1A antisera. Both antisera detected the same number of antigenic components when reacted against BBM or Fx1A preparations. When antibody was eluted from the kidneys of rats with Heymann nephritis it reacted in vitro with the renal BBM as shown by indirect immunofluorescence. Eluted antibody also cross reacted with epididymal tubules, but not with other epithelia, showing that the pathogenic antigens have a restricted tissue distribution. Heymann nephritis was readily induced in rats, but could not be induced in dogs, suggesting a species difference in susceptibility to this form of nephritis. Injection of heterologous anti-BBM or anti-Fx1A antisera resulted in rapid localization to the GBM in a granular pattern, suggesting reaction with circulating antigen or in situ immune complex formation with native or planted glomerular components. Rats with aminonucleoside nephrosis showed BBM localization of injected heterologous antisera, demonstrating their specificity in vivo. Our data provide a rationale for using purified BBM antigens for the production of a homogeneous model of Heymann nephritis.
将高度纯化的刷状缘膜（BBM）抗原在海曼肾炎中的致病性与粗制肾小管Fx1A制剂的致病性进行了比较，发现它们具有同等的致病性。两种抗原制剂均诱导了自体抗血清的形成。双向免疫扩散研究表明，抗BBM和抗Fx1A抗血清之间存在同一性反应。当与BBM或Fx1A制剂反应时，两种抗血清检测到的抗原成分数量相同。用间接免疫荧光法显示，当从患有海曼肾炎的大鼠肾脏中洗脱抗体时，它在体外与肾脏BBM发生反应。洗脱的抗体也与附睾小管发生交叉反应，但不与其他上皮组织发生反应，表明致病性抗原具有受限的组织分布。海曼肾炎很容易在大鼠中诱发，但在狗中则不能诱发，这表明对这种形式的肾炎的易感性存在物种差异。注射异种抗BBM或抗Fx1A抗血清会导致其以颗粒状模式迅速定位于肾小球基底膜（GBM），这表明与循环抗原发生反应或与天然或植入的肾小球成分形成原位免疫复合物。患有氨基核苷肾病的大鼠显示注射的异种抗血清定位于BBM，证明了它们在体内的特异性。我们的数据为使用纯化的BBM抗原制备海曼肾炎的同质模型提供了理论依据。
