Cornell R P
Am J Physiol. 1981 Mar;240(3):G225-31. doi: 10.1152/ajpgi.1981.240.3.G225.
In contrast to previous studies of neutrophils from diabetic animals and humans in vitro and of macrophages from diabetic humans in vivo, which reported phagocytic depression, reticuloendothelial system (RES) hyperphagocytosis of colloidal carbon was observed in rats at 14 and 28 days after diabetes induction with streptozotocin (STZ). Carbon clearance half times were significantly enhanced to 6.3 +/- 0.79 and 8.1 +/- 1.04 min at 14 and 28 days post-STZ, respectively, compared with the nondiabetic value (12.7 +/- 0.98 min). The severity of uncontrolled STZ-induced diabetes in rats was confirmed by significant hypoinsulinemia, hyperglucagonemia, hyperglycemia, and hyperlipidemia. Although body weights of STZ-diabetic animals declined progressively, liver weights as a percent of body weight increased above the control value at 14 and 28 days post-STZ. In fact, expression of carbon phagocytosis as the corrected phagocytic index, which accounts for changes in liver and spleen weights relative to body weight, eliminated the significant difference between STZ-diabetic and nondiabetic animals. Antibiotic treatment of diabetic rats failed to alter the hyperphagocytosis, implying that a chronic bacterial infection was not the cause of phagocytic stimulation. Daily insulin replacements, but not a single large insulin dose to 14-day post-STZ rats, reversed the enhanced phagocytosis of colloidal carbon.
与先前对糖尿病动物和人类中性粒细胞的体外研究以及糖尿病人类巨噬细胞的体内研究不同，先前的研究报告了吞噬功能下降，而在用链脲佐菌素（STZ）诱导糖尿病后的第14天和第28天，在大鼠中观察到网状内皮系统（RES）对胶体碳的吞噬作用增强。与非糖尿病值（12.7±0.98分钟）相比，STZ注射后第14天和第28天的碳清除半衰期分别显著提高至6.3±0.79分钟和8.1±1.04分钟。大鼠中未控制的STZ诱导糖尿病的严重程度通过显著的低胰岛素血症、高胰高血糖素血症、高血糖症和高脂血症得到证实。尽管STZ糖尿病动物的体重逐渐下降，但在STZ注射后第14天和第28天，肝脏重量占体重的百分比高于对照值。事实上，以校正吞噬指数表示的碳吞噬作用，该指数考虑了肝脏和脾脏重量相对于体重的变化，消除了STZ糖尿病动物和非糖尿病动物之间的显著差异。对糖尿病大鼠进行抗生素治疗未能改变吞噬作用增强的情况，这意味着慢性细菌感染不是吞噬刺激的原因。每日胰岛素替代治疗，而不是对STZ注射后14天的大鼠单次给予大剂量胰岛素，可逆转胶体碳吞噬作用增强的情况。
