Dumon H, Manoury B, Quilici M, Ranque J
C R Seances Soc Biol Fil. 1981;175(1):82-6.
It has been studied the continuous growth of a Leishmania infantum canine strain from Marseille. The authors used a modified liquid medium with Panmed : foetal calf serum and calf serum. They used a bench to chemistat at 24 degrees C. With an optimum concentration inoculum they obtained a diphasic growth curve with a first exponential phase from the third to the fifth day, in which the average time of generation is 15 hours and 20 minutes, and a second growth phase from the ninth to the eleventh day. It is a--diauxie growth--(according to the J. Monod Theory). If some medium is added on the third day this first exponential growth phase continues up to the eleventh day.
对来自马赛的一株婴儿利什曼原虫犬株的持续生长进行了研究。作者使用了一种添加泛美霉素的改良液体培养基:胎牛血清和小牛血清。他们在24℃下使用台式恒化器。在最佳接种浓度下,他们获得了双相生长曲线,第一个指数生长期从第三天到第五天,其中平均世代时间为15小时20分钟,第二个生长阶段从第九天到第十一天。这是一种双期生长(根据J.莫诺德理论)。如果在第三天添加一些培养基,这个第一个指数生长阶段会持续到第十一天。