果糖2,6-二磷酸，可能是磷酸果糖激酶的葡萄糖和胰高血糖素敏感刺激物的结构。
Fructose 2,6-bisphosphate, the probably structure of the glucose- and glucagon-sensitive stimulator of phosphofructokinase.
作者信息
Van Schaftingen E, Hue L, Hers H G
出版信息
Biochem J. 1980 Dec 15;192(3):897-901. doi: 10.1042/bj1920897.
Abstract
The low-molecular-weight stimulator of phosphofructokinase [Van Schaftingen, Hue & Hers (1980) Biochem. J. 192, 887-895] has been purified from rat liver. It was completely destroyed upon incubation with 0.01 M-HCl for 10 min at 20 degrees C and fructose 6-phosphate and a reducing power equivalent in amount to the acid-labile organic phosphate were formed. It was therefore tentatively identified as fructose 2,6-bisphosphate.
摘要
磷酸果糖激酶的低分子量刺激物[范·沙夫廷根、休和赫尔斯（1980年）《生物化学杂志》192卷，887 - 895页]已从大鼠肝脏中纯化出来。在20℃下与0.01 M - HCl孵育10分钟后，它被完全破坏，并形成了6 - 磷酸果糖和与酸不稳定有机磷酸等量的还原力。因此，初步鉴定其为2,6 - 二磷酸果糖。
相似文献
1
Fructose 2,6-bisphosphate, the probably structure of the glucose- and glucagon-sensitive stimulator of phosphofructokinase.果糖2,6-二磷酸，可能是磷酸果糖激酶的葡萄糖和胰高血糖素敏感刺激物的结构。
Biochem J. 1980 Dec 15;192(3):897-901. doi: 10.1042/bj1920897.
2
Control of the fructose-6-phosphate/fructose 1,6-bisphosphate cycle in isolated hepatocytes by glucose and glucagon. Role of a low-molecular-weight stimulator of phosphofructokinase.葡萄糖和胰高血糖素对分离肝细胞中磷酸果糖-6-磷酸/果糖1,6-二磷酸循环的调控。磷酸果糖激酶低分子量刺激物的作用。
Biochem J. 1980 Dec 15;192(3):887-95. doi: 10.1042/bj1920887.
3
Evidence for a new activator of rat liver phosphofructokinase.大鼠肝脏磷酸果糖激酶新激活剂的证据。
Biochem Biophys Res Commun. 1981 Jan 30;98(2):359-66. doi: 10.1016/0006-291x(81)90848-2.
4
Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-biphosphate.果糖2,6-二磷酸对果糖-1,6-二磷酸酶的抑制作用。
Proc Natl Acad Sci U S A. 1981 May;78(5):2861-3. doi: 10.1073/pnas.78.5.2861.
5
6
Control of liver 6-phosphofructokinase by fructose 2,6-bisphosphate and other effectors.果糖2,6-二磷酸及其他效应物对肝脏6-磷酸果糖激酶的调控
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3483-6. doi: 10.1073/pnas.78.6.3483.
7
Hormonal control of fructose 2,6-bisphosphate concentration and of phosphofructokinase 2 in the rat liver during development.发育过程中大鼠肝脏中果糖-2,6-二磷酸浓度及磷酸果糖激酶-2的激素调控
Biosci Rep. 1986 Jun;6(6):513-8. doi: 10.1007/BF01114947.
8
Stimulation of glycolysis and accumulation of a stimulator of phosphofructokinase in hepatocytes incubated with vasopressin.用血管加压素孵育肝细胞时，糖酵解的刺激及磷酸果糖激酶刺激物的积累。
Biochem J. 1981 Mar 15;194(3):1023-6. doi: 10.1042/bj1941023.
9
Activation of phosphofructokinase from rat tissues by 6-phosphogluconate and fructose 2,6-bisphosphate.6-磷酸葡萄糖酸和果糖2,6-二磷酸对大鼠组织中磷酸果糖激酶的激活作用。
Arch Biochem Biophys. 1984 Aug 1;232(2):579-84. doi: 10.1016/0003-9861(84)90576-9.
10
Inorganic phosphate amplifies the effects of AMP and fructose-2,6-bisphosphate on yeast phosphofructokinase.无机磷酸盐会增强AMP和果糖-2,6-二磷酸对酵母磷酸果糖激酶的作用。
Biomed Biochim Acta. 1985;44(11-12):1559-65.
引用本文的文献
1
SIRT3 deficiency reduces PFKFB3-driven T-cell glycolysis and promotes arthritic inflammation.SIRT3缺乏会降低PFKFB3驱动的T细胞糖酵解，并促进关节炎炎症。
Sci China Life Sci. 2025 Jun;68(6):1755-1769. doi: 10.1007/s11427-024-2823-2. Epub 2025 Feb 27.
2
PFKFB3-dependent redox homeostasis and DNA repair support cell survival under EGFR-TKIs in non-small cell lung carcinoma.在非小细胞肺癌中，磷酸果糖激酶-2/果糖-2,6-二磷酸酶3（PFKFB3）依赖性氧化还原稳态和DNA修复支持表皮生长因子受体酪氨酸激酶抑制剂（EGFR-TKIs）作用下的细胞存活。
Cancer Metab. 2024 Dec 18;12(1):37. doi: 10.1186/s40170-024-00366-y.
3
Genomic Deletion of PFKFB3 Decreases In Vivo Tumorigenesis.PFKFB3的基因组缺失降低体内肿瘤发生。
Cancers (Basel). 2024 Jun 26;16(13):2330. doi: 10.3390/cancers16132330.
4
Hitting the Sweet Spot: How Glucose Metabolism Is Orchestrated in Space and Time by Phosphofructokinase-1.找到关键所在：磷酸果糖激酶-1如何在空间和时间上协调葡萄糖代谢
Cancers (Basel). 2023 Dec 19;16(1):16. doi: 10.3390/cancers16010016.
5
Metabolites as signalling molecules.作为信号分子的代谢物
Nat Rev Mol Cell Biol. 2023 May;24(5):355-374. doi: 10.1038/s41580-022-00572-w. Epub 2023 Jan 12.
6
The role of PFKFB3 in maintaining colorectal cancer cell proliferation and stemness.PFKFB3 在维持结直肠癌细胞增殖和干性中的作用。
Mol Biol Rep. 2022 Oct;49(10):9877-9891. doi: 10.1007/s11033-022-07513-y. Epub 2022 May 12.
7
Treatment against glucose-dependent cancers through metabolic PFKFB3 targeting of glycolytic flux.通过靶向糖酵解通量的代谢 PFKFB3 治疗葡萄糖依赖性癌症。
Cancer Metastasis Rev. 2022 Jun;41(2):447-458. doi: 10.1007/s10555-022-10027-5. Epub 2022 Apr 14.
8
Sex differences in metabolic pathways are regulated by Pfkfb3 and Pdk4 expression in rodent muscle.在啮齿类动物肌肉中， Pfkfb3 和 Pdk4 的表达调控着代谢途径的性别差异。
Commun Biol. 2021 Nov 4;4(1):1264. doi: 10.1038/s42003-021-02790-y.
9
PFKFB3 Inhibition Impairs Erlotinib-Induced Autophagy in NSCLCs.PFKFB3 抑制可削弱 NSCLC 中厄洛替尼诱导的自噬。
Cells. 2021 Jul 3;10(7):1679. doi: 10.3390/cells10071679.
10
Determining the quantitative relationship between glycolysis and GAPDH in cancer cells exhibiting the Warburg effect.测定表现出瓦博格效应的癌细胞中糖酵解和 GAPDH 之间的定量关系。
J Biol Chem. 2021 Jan-Jun;296:100369. doi: 10.1016/j.jbc.2021.100369. Epub 2021 Feb 3.
本文引用的文献
1
A submicrodetermination of glucose.葡萄糖的亚微量测定
J Biol Chem. 1949 Nov;181(1):149-51.
2
Some properties of the phosphoribomutase reaction.磷酸核糖变位酶反应的一些特性。
Arch Biochem Biophys. 1953 Sep;46(1):186-200. doi: 10.1016/0003-9861(53)90181-7.
3
Control of the fructose-6-phosphate/fructose 1,6-bisphosphate cycle in isolated hepatocytes by glucose and glucagon. Role of a low-molecular-weight stimulator of phosphofructokinase.葡萄糖和胰高血糖素对分离肝细胞中磷酸果糖-6-磷酸/果糖1,6-二磷酸循环的调控。磷酸果糖激酶低分子量刺激物的作用。
Biochem J. 1980 Dec 15;192(3):887-95. doi: 10.1042/bj1920887.
4
A new micromethod for the colorimetric determination of inorganic phosphate.一种用于比色法测定无机磷酸盐的新微量法。
Clin Chim Acta. 1966 Sep;14(3):361-6. doi: 10.1016/0009-8981(66)90114-8.
5
Hormone-stimulated phosphorylation of liver phosphofructokinase in vivo.体内激素刺激的肝脏磷酸果糖激酶磷酸化作用
J Biol Chem. 1979 Jul 10;254(13):5584-7.
6
Regulation of phosphofructokinase activity by glucagon in isolated rat hepatocytes.胰高血糖素对离体大鼠肝细胞中磷酸果糖激酶活性的调节作用。
Biochem Biophys Res Commun. 1979 Jun 13;88(3):960-7. doi: 10.1016/0006-291x(79)91501-8.
7
Inactivation of phosphofructokinase by glucagon in rat hepatocytes.胰高血糖素对大鼠肝细胞中磷酸果糖激酶的失活作用。
J Biol Chem. 1979 Jul 10;254(13):5576-9.
Zotero 插件