Kolár F, Mejsnar J
Physiol Bohemoslov. 1981;30(2):123-8.
Vascularly isolated skeletal muscle of the cold-acclimated (CA) rat was perfused with blood in situ or in vitro and the effect of denervation and an alpha-adrenolytic agent (phentolamine) on its oxygen consumption was studied in the resting state and after administering noradrenaline (NA). The resting metabolism of muscle in situ rose by 28% after denervation. The infusion of NA further raised the oxygen consumption of acutely denervated muscle perfused in situ of in vitro by 43%. The thermogenic effect of NA on muscle denervated two hours before the experiment was only transitory. Phentolamine raised the oxygen consumption of the innervated muscle in situ by 42%; the infusion of NA did not stimulate metabolism any further. Phentolamine reduced the vascular resistance of resting muscle, but did not inhibit the vasoconstriction during the infusion of NA. The results show that the thermogenic effect of infused NA in perfused muscle is inhibited not by acute denervation, but by a vasoconstriction, which cannot be prevented by the administration of an alpha-adrenolytic agent.
对冷适应（CA）大鼠的血管分离骨骼肌进行原位或体外血液灌注，并研究去神经支配和α-肾上腺素能阻滞剂（酚妥拉明）对其在静息状态下以及给予去甲肾上腺素（NA）后的耗氧量的影响。去神经支配后，原位肌肉静息代谢率升高了28%。输注NA进一步使原位或体外灌注的急性去神经支配肌肉的耗氧量提高了43%。NA对实验前两小时去神经支配的肌肉的产热作用只是暂时的。酚妥拉明使原位有神经支配的肌肉的耗氧量提高了42%；输注NA不再进一步刺激代谢。酚妥拉明降低了静息肌肉的血管阻力，但不抑制输注NA期间的血管收缩。结果表明，灌注肌肉中输注NA的产热作用不是被急性去神经支配所抑制，而是被血管收缩所抑制，而给予α-肾上腺素能阻滞剂并不能防止这种血管收缩。
