Orsetti A, Brun J F, Bouhaddioui N, Crespy S
C R Seances Soc Biol Fil. 1981;175(2):221-7.
Six dogs received intraportally an autograft of isolated non purified pancreatic islets after total pancreatectomy. The isolation was made by collagenase technique, using a quarter to a half of the total pancreatic mass. The results of different transplantation technics are discussed. We conclude the necessity of: 1) a good venous distension of the pancreas, 2) the use of at least a third of the pancreatic gland to prepare the islets, 3) a tissue reinjection the closest as possible of the portal vein.
六只狗在全胰切除术后经门静脉接受了未纯化的分离胰岛自体移植。采用胶原酶技术进行分离,使用了胰腺总体积的四分之一到二分之一。讨论了不同移植技术的结果。我们得出以下必要性结论:1)胰腺要有良好的静脉扩张;2)使用至少三分之一的胰腺组织来制备胰岛;3)尽可能将组织重新注入门静脉附近。