膜蛋白掺入脂质体的研究——二价阳离子对猪心脏线粒体H⁺-ATP酶重组到脂质体中的影响。
Studies on incorporation of membrane protein into liposomes--effect of divalent cations on reconstitution of pig heart mitochondrial H+-ATPase into liposomes.
作者信息
Yang F Y, Guo B Q, Huang Y G, Che Y W
出版信息
Sci Sin. 1981 Jul;24(7):989-96.
During the process of reconstitution of pig heart mitochondrial H+-ATPase on liposomes by cholate dialysis method, 1 mM Mg2+ in the dialysis medium can obviously increase activities of the reconstituted H+-ATPase (32Pi-ATP exchange, enzyme activity and its sensitivity to oligomycin or DCCD and ATP-dependent ANS 1) fluorescence). Besides Mg2+, effects of other divalent cations and spermidine on the H+-ATPase reconstitution have been compared. The effectiveness of the divalent cation activation of 32Pi-ATP exchange of the reconstituted H+-ATPase, decreased in the order: Mg2+ greater than Ca2+ greater than Mn2+ greater than Sr2+, while that of increasing the sensitivity to the inhibitors: Ca2+ greater than Mg2+ greater than Mn2+ greater than Sr2+. No significant effect on the H+-ATPase reconstitution has been found with Cd2+, Zn2+ and spermidine3+. Mechanism of action of the divalent cations on reconstitution has been discussed.
在用胆酸盐透析法将猪心脏线粒体H⁺-ATP酶重组到脂质体上的过程中，透析介质中的1 mM Mg²⁺能显著提高重组H⁺-ATP酶的活性（³²Pi-ATP交换、酶活性及其对寡霉素或二环己基碳二亚胺的敏感性以及ATP依赖的ANS荧光）。除了Mg²⁺，还比较了其他二价阳离子和亚精胺对H⁺-ATP酶重组的影响。重组H⁺-ATP酶³²Pi-ATP交换的二价阳离子激活效果顺序为：Mg²⁺＞Ca²⁺＞Mn²⁺＞Sr²⁺，而增加对抑制剂敏感性的顺序为：Ca²⁺＞Mg²⁺＞Mn²⁺＞Sr²⁺。未发现Cd²⁺、Zn²⁺和亚精胺对H⁺-ATP酶重组有显著影响。讨论了二价阳离子对重组的作用机制。
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