Tauber J P, Cheng J, Massoglia S, Gospodarowicz D
In Vitro. 1981 Jun;17(6):519-30. doi: 10.1007/BF02633513.
Low density bovine vascular endothelial cell cultures maintained on dishes coated with an extracellular matrix can be grown in serum-free Dulbecco's modified Eagle's medium supplemented with high density lipoprotein (HDL) and transferrin. Such cultures do not require insulin. Early passage cultures exposed to HDL and transferrin grew as well as cultures exposed to optimal serum concentrations and could be passaged repeatedly in total absence of serum. A requirement for fibroblast growth factor to ensure an optimal growth could be observed only with late-passage cultures. The present results suggest strongly that HDL is involved in supporting the proliferation of vascular endothelial cells in vitro. This may be important for our understanding of the biological role of HDL "in vivo."
在涂有细胞外基质的培养皿上维持的低密度牛血管内皮细胞培养物，可以在添加了高密度脂蛋白（HDL）和转铁蛋白的无血清杜尔贝科改良伊格尔培养基中生长。这种培养物不需要胰岛素。暴露于HDL和转铁蛋白的早期传代培养物生长情况与暴露于最佳血清浓度的培养物相同，并且可以在完全无血清的情况下反复传代。仅在晚期传代培养物中才能观察到对成纤维细胞生长因子的需求以确保最佳生长。目前的结果强烈表明，HDL参与支持体外血管内皮细胞的增殖。这对于我们理解HDL“体内”的生物学作用可能很重要。
