Dall Pai V D, Santos Costa I R, Pacheco A C, Alves C E, Macha N
Folia Histochem Cytochem (Krakow). 1981;19(2):107-13.
Histochemical study of mucopolysaccharides of both mucous secreting and subjacent granular cells of the epidermis of Glossoscolex uruguayensis L (Righi, 1978), has been done. Histochemical tests revealed two major types of mucous cells: large ortochromatic mucous cells and metachromatic mucous cells. The first ones possess alpha-amylase resistant, PAS-positive material, together with little mucic acid, and the latter present a very low rate of neutral mucopolysaccharides and to a variable degree, both carboxylate and sulphate mucopolysaccharides. A third cell type was also detected, presenting a frothy acid like material. Subjacent epidermal granular cells display alpha-amylase resistant, PAS-positive granules, with no acid mucus.
对乌拉圭舌蛭(Righi,1978)表皮的黏液分泌细胞和下层颗粒细胞的黏多糖进行了组织化学研究。组织化学测试揭示了两种主要类型的黏液细胞:大型正染黏液细胞和异染黏液细胞。前者含有抗α淀粉酶、PAS阳性物质以及少量黏液酸,而后者的中性黏多糖含量极低,同时含有不同程度的羧化和硫酸化黏多糖。还检测到第三种细胞类型,呈现出泡沫状酸性物质。下层表皮颗粒细胞显示出抗α淀粉酶、PAS阳性颗粒,无酸性黏液。