通过微型转导将鼠伤寒沙门氏菌和肺炎克雷伯菌的基因转移至大肠杆菌K12中。
Transfer of Salmonella typhimurium and Klebsiella pneumoniae genes in E. coli K12 by mini-muduction.
作者信息
Lefèbvre N, Toussaint A
出版信息
Mol Gen Genet. 1981;181(2):268-72. doi: 10.1007/BF00268436.
PMID:6456401
Abstract
Using Mu/mini-Mu mixed lysates prepared by induction of Salmonella typhimurium and Klebsiella pneumoniae lysogenic for Mucts62 or the Mu-P1 hybrid, MPh1, and mini-Mu, Mu18A-1, we were able to transfer Arg and His genes from Klebsiella and Salmonella into E. coli K12.
摘要
利用通过诱导对Mucts62或Mu-P1杂种MPh1溶原的鼠伤寒沙门氏菌和肺炎克雷伯菌制备的Mu/mini-Mu混合裂解物，以及mini-Mu Mu18A-1，我们能够将来自克雷伯菌和沙门氏菌的精氨酸和组氨酸基因转移到大肠杆菌K12中。
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