Koyama S, Fujimoto S, Tada T, Sakita T
Int J Cancer. 1981 Jun 15;27(6):829-35. doi: 10.1002/ijc.2910270615.
The effect of Bacillus Calmette-Guérin cell wall skeleton (BCG-CWS), which is one of the well-established immunopotentiators, on the induction of either cytotoxic or suppressor T cells against syngeneic tumor S1509a was investigated by an in vitro assay system. BCG-CWS showed an apparent augmenting activity in the induction of cytotoxic T cells against a syngeneic tumor when it was administered subcutaneously into mice with mitomycin C (MMC)-treated tumor cells in a form of oil-in-water emulsion. In addition, cytotoxic T cell activity was dominantly generated in regional lymph nodes. Furthermore, the immunized animals developed stronger resistance against the second inoculum of the same viable tumor cells. However, BCG-CWS had no effect on the induction of suppressor T cells in tumor-bearing host (TBH), and could not induce cytotoxic T cells against the homologous tumor in TBH in which suppressor T cells had been rapidly activated. The growth of tumor was neither suppressor nor enhanced, even if an oil-attached BCG-CWS was administered in TBH.
卡介苗细胞壁骨架（BCG-CWS）是一种公认的免疫增强剂，本研究通过体外检测系统，研究了其对诱导同基因肿瘤S1509a的细胞毒性T细胞或抑制性T细胞的影响。当以水包油乳剂形式将BCG-CWS与丝裂霉素C（MMC）处理的肿瘤细胞皮下注射到小鼠体内时，BCG-CWS在诱导针对同基因肿瘤的细胞毒性T细胞方面表现出明显的增强活性。此外，细胞毒性T细胞活性主要在局部淋巴结中产生。此外，免疫动物对相同活肿瘤细胞的第二次接种产生了更强的抵抗力。然而，BCG-CWS对荷瘤宿主（TBH）中抑制性T细胞的诱导没有影响，并且在抑制性T细胞已被快速激活的TBH中不能诱导针对同源肿瘤的细胞毒性T细胞。即使在TBH中给予油附BCG-CWS，肿瘤的生长既没有受到抑制也没有增强。
