Chang T W, Gingras S P
Int J Immunopharmacol. 1981;3(3):183-6. doi: 10.1016/0192-0561(81)90011-4.
Human alloreactive cytotoxic T lymphocytes (CTLs) were generated in culture by stimulating peripheral blood lymphocytes against lymphocytes of unrelated donors. OKT3 monoclonal antibody, which reacts with all human peripheral T cells, inhibited more than 50% of the lysis of the allogeneic cells by the CTLs in the four-hour cytotoxicity assay at a concentration of 1 microgram ml-1. OKT4, which reacts with inducer/helper T cells, did not demonstrate this effect. OKT5 and OKT8, both of which react with cytotoxic/suppressor T cells, had a small but distinct inhibitory effect (about 20-30% maximally). OKT3 antibody is also known to induce mitogenesis of peripheral T cells, but this mitogenic effect is probably of itself not the cause of the inhibitory effect of OKT3 on CTLs. Human plasma abolished OKT3-induced mitogenesis of T cells but not OKT3-mediated inhibition of CTL activity. In addition, there exist conditions that allow both full mitogenesis and intact CTL activity, suggesting CTLs can maintain cytolytic activity under mitogenic conditions. The generation of CTLs in the 6-day mixed lymphocyte culture was also inhibited by OKT3. Several possible mechanisms for this effect are discussed.
通过用无关供体的淋巴细胞刺激外周血淋巴细胞，在培养中产生人同种异体反应性细胞毒性T淋巴细胞（CTL）。与所有人外周T细胞发生反应的OKT3单克隆抗体，在浓度为1微克/毫升时，在四小时细胞毒性试验中抑制了CTL对同种异体细胞的50%以上的裂解。与诱导/辅助性T细胞发生反应的OKT4没有显示出这种作用。与细胞毒性/抑制性T细胞发生反应的OKT5和OKT8有微小但明显的抑制作用（最大约20%-30%）。已知OKT3抗体也可诱导外周T细胞的有丝分裂，但这种有丝分裂作用本身可能不是OKT3对CTL抑制作用的原因。人血浆消除了OKT3诱导的T细胞有丝分裂，但没有消除OKT3介导的CTL活性抑制。此外，存在允许完全有丝分裂和完整CTL活性的条件，这表明CTL在有丝分裂条件下可维持细胞溶解活性。OKT3也抑制了6天混合淋巴细胞培养中CTL的产生。讨论了这种作用的几种可能机制。
