Lau C, Goldstein G
Int J Immunopharmacol. 1981;3(3):187-92. doi: 10.1016/0192-0561(81)90012-6.
Human peripheral lymphocytes pretreated with the Orthoclone monoclonal anti-T cell antibody OKT3 for 48 h markedly suppressed the proliferative response of autologous lymphocytes in one-way MLC and the mitogenic response to PHA. The ability to induce suppression is specific to OKT3 since other monoclonal antibodies to human T cells (OKT1, OKT4 and OKT8) did not elicit similar responses, OKT3 is mitogenic but further proliferation of OKT3 pretreated lymphocytes was not required for the suppression of autologous lymphocytes since mitomycin-C treated cells were fully effective. Kinetic studies indicated that pretreatment of lymphocytes with OKT3 for 24 h was sufficient to induce marked inhibition of the mitogenic response of autologous lymphocytes to PHA whereas suppression in MLC was not observed until lymphocytes were pretreated for 48 h. These studies support the previous observations that OKT3 may be reacting with an important molecule on the T cell surface and that interaction of OKT3 with this molecule induces profound functional changes.
用Orthoclone单克隆抗T细胞抗体OKT3预处理人外周血淋巴细胞48小时，可显著抑制单向混合淋巴细胞培养中自体淋巴细胞的增殖反应以及对PHA的促有丝分裂反应。诱导抑制的能力对OKT3具有特异性，因为其他针对人T细胞的单克隆抗体（OKT1、OKT4和OKT8）未引发类似反应。OKT3具有促有丝分裂作用，但OKT3预处理的淋巴细胞进一步增殖并非抑制自体淋巴细胞所必需，因为丝裂霉素C处理的细胞同样有效。动力学研究表明，用OKT3预处理淋巴细胞24小时足以显著抑制自体淋巴细胞对PHA的促有丝分裂反应，而在单向混合淋巴细胞培养中，直到淋巴细胞预处理48小时才观察到抑制作用。这些研究支持了先前的观察结果，即OKT3可能与T细胞表面的一种重要分子发生反应，且OKT3与该分子的相互作用会引发深刻的功能变化。
