Synthesis and assembly of adenosinetriphosphatase in synchronous cultures of yeast.

Maximal respiration and expression of mitochondrial enzymes are found at the late-S phase of yeast cells growing synchronously in glucose medium. Adenosinetriphosphatase (ATPase) activity follows a similar pattern. However, the cytosolically synthesized F1-ATPase and also that released from the membrane accumulate in the cytosol during the G1 and early-S phases. After the mid-S phase, when the mitochondrially synthesized membrane factors are available, the enzyme migrates to the membrane and is integrated.