Kunicki T J, Nurden A T, Pidard D, Russell N R, Caen J P
Blood. 1981 Dec;58(6):1190-7.
Washed human platelets were labeled with 125I by the lactoperoxidase-catalyzed method and solubilized in 1% Triton X-100. The soluble proteins were analyzed by crossed-immunoelectrophoresis in 1% agarose, employing a rabbit antibody raised against whole human platelets. Analysis of autoradiograms developed from dried agarose gels led to the establishment of a normal reference pattern that was consistent for platelets obtained from more than 50 normal individuals. Six platelet membrane glycoprotein antigens contained in four distinguishable precipitates were identified. Each identification was based on direct sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of 125I-antigens contained in individually excised precipitates. These platelet antigens include major membrane glycoproteins previously designated la, lb, lla, llb, llla, and lllb. Glycoproteins llb and llla were shown to be contained in a single immunoprecipitate, while glycoproteins la and lla were routinely detected in a single different immunoprecipitate. Analysis of soluble proteins from platelets of five patients with Glanzmann's thrombasthenia demonstrated either a complete absence or a marked reduction of only one radiolabeled precipitate, that containing membrane glycoproteins llb and llla. Platelet samples from two patients with Bernard-Soulier syndrome were devoid of a different precipitate, that containing membrane glycoprotein lb.
采用乳过氧化物酶催化法，用125I对洗涤后的人血小板进行标记，然后将其溶解于1%的 Triton X - 100中。将可溶性蛋白质在1%的琼脂糖中通过交叉免疫电泳进行分析，使用的是针对整个人血小板产生的兔抗体。对干燥琼脂糖凝胶显影后的放射自显影片进行分析，得出了一个正常参考图谱，该图谱对于从50多名正常个体获得的血小板是一致的。在四个可区分的沉淀中鉴定出六种血小板膜糖蛋白抗原。每次鉴定均基于对单个切下的沉淀中所含125I抗原进行的直接十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析。这些血小板抗原包括先前命名为la、lb、lla、llb、llla和lll b的主要膜糖蛋白。糖蛋白llb和llla显示包含在单个免疫沉淀中，而糖蛋白la和lla通常在另一个不同的免疫沉淀中检测到。对五名血小板无力症患者的血小板可溶性蛋白质进行分析，结果显示要么完全没有，要么只有一种放射性标记沉淀（即包含膜糖蛋白llb和llla的沉淀）显著减少。两名伯纳德 - 索利尔综合征患者的血小板样本缺少另一种沉淀，即包含膜糖蛋白lb的沉淀。
