Schmitt M, Mussel H H, Dierich M P
J Immunol Methods. 1981;47(1):75-85. doi: 10.1016/0022-1759(81)90258-1.
Particle-bound human C3b (EAC14oxy23b) serves as the carrier for the detection of biologically active human beta 1 H by IgG-anti-beta 1H. This hemagglutination assay is highly sensitive (greater than or equal to 2 p of beta 1H/40 microliters), easy to perform, rapid, highly reproducible, and needs no expensive laboratory equipment. All reagents are commercially available. The correlation of the beta 1H-hemagglutination assay is applicable for the detection of beta 1H in various buffer systems and may be used for the detection of beta 1H in serum or fractions thereof. It is a very useful tool for the detection of trace contamination of beta 1H in serum fractions, particularly in complement preparations.
结合颗粒的人C3b（EAC14oxy23b）作为载体，用于通过IgG抗β1H检测具有生物活性的人β1H。这种血凝试验高度灵敏（≥2 pβ1H/40微升），易于操作，快速，重复性高，且无需昂贵的实验室设备。所有试剂均可从市场购得。β1H血凝试验的相关性适用于在各种缓冲系统中检测β1H，也可用于检测血清或其组分中的β1H。它是检测血清组分中β1H微量污染的非常有用的工具，特别是在补体制剂中。
