亨廷顿舞蹈病成纤维细胞中UDP-N-乙酰葡糖胺的差异标记
Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts.
作者信息
Hung W Y, Tourian A
出版信息
Biochem J. 1981 May 15;196(2):495-8. doi: 10.1042/bj1960495.
Abstract
The hypothesis that there is impaired endogenous synthesis of glucosamine 6-phosphate in Huntington's-chorea fibroblasts was tested by double labelling matched pairs of fibroblasts in culture with carrier-free H3 32PO4 and [U-14C]glucosamine. The [32P]UDP-N-acetyl[14C]glucosamine and [14C]glucosamine 6-[32P]phosphate of the cellular soluble fraction was isolated by charcoal column and paper chromatography. There is no quantitative difference in 32P but a significant difference in 14C in these two sugars in a ratio of approx. 1.5 for Huntington's-chorea fibroblasts compared with normal fibroblasts.
摘要
通过用无载体的H3 32PO4和[U-14C]葡糖胺对培养的亨廷顿舞蹈病成纤维细胞配对进行双重标记，来检验亨廷顿舞蹈病成纤维细胞中6-磷酸葡糖胺内源性合成受损这一假说。细胞可溶性部分的[32P]UDP-N-乙酰基[14C]葡糖胺和[14C]6-[32P]磷酸葡糖胺通过活性炭柱和纸色谱法分离。在这两种糖中，亨廷顿舞蹈病成纤维细胞与正常成纤维细胞相比，32P没有定量差异，但14C存在显著差异，比例约为1.5。
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