Cunningham D S, Collins F M
Immunology. 1981 Nov;44(3):473-80.
Specific pathogen-free B6D2 F1 hybrid mice were infected intravenously with increasing numbers of Mycobacterium kansasii TMC 1203 or 1214 and blastogenic responsiveness of the splenic T cells to phytohaemagglutinin (PHA), Con A or to a mixed lymphocyte reaction (MLR) were compared. Mice which had been infected 30 days earlier with M. kansasii strain 1203 exhibited substantial suppression whereas those infected with 1214 showed enhanced tritiated thymidine uptakes when exposed to mitogenic or allogeneic stimulation. Spleen cells harvested from mice infected 10 days earlier with either M. kansasii 1203 or 1214 exhibited substantially enhanced levels of helper T-cell activity in vitro compared with normal spleen cells. As the M. kansasii 1203 infections progressed, increasing suppressor T-cell activity was observed so that by day 30, the helper T-cell response had been largely ablated. On the other hand, the M. kansasii 1214-infected spleens exhibited little or no suppressor T-cell activity at any time during the infection. However, helper T-cell activity doubled over this same time period relative to that for the 1203-infected spleens when the responses were compared on a cell-to-cell basis.
将数量不断增加的堪萨斯分枝杆菌TMC 1203或1214静脉注射到无特定病原体的B6D2 F1杂交小鼠体内，比较脾脏T细胞对植物血凝素（PHA）、刀豆蛋白A（Con A）或混合淋巴细胞反应（MLR）的增殖反应性。30天前感染堪萨斯分枝杆菌1203株的小鼠表现出明显的抑制作用，而感染1214株的小鼠在受到促有丝分裂或同种异体刺激时，氚化胸腺嘧啶核苷摄取量增加。与正常脾细胞相比，10天前感染堪萨斯分枝杆菌1203或1214的小鼠收获的脾细胞在体外表现出明显增强的辅助性T细胞活性水平。随着堪萨斯分枝杆菌1203感染的进展，观察到抑制性T细胞活性增加，以至于到第30天，辅助性T细胞反应已基本被消除。另一方面，感染堪萨斯分枝杆菌1214的脾脏在感染期间的任何时候都表现出很少或没有抑制性T细胞活性。然而，当在细胞对细胞的基础上比较反应时，在同一时间段内，相对于感染1203的脾脏，感染1214的脾脏的辅助性T细胞活性增加了一倍。
