采用高效液相色谱法测定丁苯那嗪及其活性代谢物的治疗性血浆浓度。

Determination of therapeutic plasma concentrations of tetrabenazine and an active metabolite by high-performance liquid chromatography.

作者信息

Roberts M S, Watson H M, McLean S, Millingen K S

出版信息

J Chromatogr. 1981 Nov 13;226(1):175-82. doi: 10.1016/s0378-4347(00)84218-8.

Abstract

A reversed-phase high-performance liquid chromatographic method for the determination of tetrabenazine and a hydroxy metabolite in plasma is described. Tetrabenazine and the hydroxy metabolite are quantified as their dehydro derivatives using fluorescence detection. This method has been applied to the analysis of plasma samples from patients with Huntington's chorea and has been found to be sensitive, reliable and specific for tetrabenazine and the hydroxy metabolite. The plasma concentrations of tetrabenazine found in patients were lower than could be detected using previously published methods.

摘要

本文描述了一种用于测定血浆中丁苯那嗪及其羟基代谢物的反相高效液相色谱法。丁苯那嗪及其羟基代谢物通过荧光检测，以其脱氢衍生物的形式进行定量。该方法已应用于亨廷顿舞蹈症患者血浆样本的分析，结果表明该方法对丁苯那嗪及其羟基代谢物具有灵敏性、可靠性和特异性。患者血浆中丁苯那嗪的浓度低于此前发表的方法所能检测到的水平。