Gandhi K K, Anand S R
J Reprod Fertil. 1982 Jan;64(1):145-50. doi: 10.1530/jrf.0.0640145.
Assay of maximal activities of 11 glycolytic enzymes in cell-free buffalo sperm extracts showed that hexokinase, phosphofructokinase and glyceraldehyde-3-phosphate dehydrogenase had the lowest activities, suggesting regulation of fructolysis at steps catalysed by these enzymes. The ratios of glyceraldehyde-3-phosphate dehydrogenase/phosphofructokinase (0.67) and phosphoglycerate kinase/phosphofructokinase (4.60) are typical of cells exhibiting high Pasteur effect (50% for ejaculated buffalo spermatozoa). The regulatory nature of phosphofructokinase was shown through its modulation by ATP, AMP and inorganic phosphate. The determination of fructolytic intermediates and cofactors and calculation of mass action ratios for each enzymic step revealed that hexokinase, phosphofructokinase, fructose-biphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase catalysed reactions far removed from the equilibrium. A regulatory role by glyceraldehyde-3-phosphate dehydrogenase appeared to be most likely because triosephosphates and inorganic phosphate accumulated more under anaerobic than under aerobic conditions.
对无细胞水牛精子提取物中11种糖酵解酶的最大活性进行分析表明，己糖激酶、磷酸果糖激酶和甘油醛-3-磷酸脱氢酶的活性最低，这表明在由这些酶催化的步骤中存在对果糖分解的调节。甘油醛-3-磷酸脱氢酶/磷酸果糖激酶的比率（0.67）和磷酸甘油酸激酶/磷酸果糖激酶的比率（4.60）是表现出高巴斯德效应的细胞的典型特征（射精的水牛精子为50%）。通过ATP、AMP和无机磷酸盐对磷酸果糖激酶的调节，表明了其调节性质。对果糖分解中间产物和辅助因子的测定以及每个酶促步骤质量作用比的计算表明，己糖激酶、磷酸果糖激酶、果糖二磷酸醛缩酶、甘油醛-3-磷酸脱氢酶和丙酮酸激酶催化的反应远未达到平衡。甘油醛-3-磷酸脱氢酶的调节作用似乎最有可能，因为在厌氧条件下比在需氧条件下磷酸丙糖和无机磷酸盐积累得更多。
