雌激素刺激后输卵管细胞组分中高迁移率族蛋白1含量的变化

Biochem J. 1981 Jul 15;198(1):85-90. doi: 10.1042/bj1980085.

Antiserum against chick oviduct high-mobility-group protein 1 (HMG 1) has been induced in the rabbit. With this antiserum, immunobiochemical techniques have been used to probe the quantitative change of HMG 1 in the cellular fractions of chick oviduct before or after oestrogen stimulation. HMG 1 is detectable in the cytosol, microsomal and nuclear fraction of the chick oviduct cell. After administration of oestrogen to young chicks in vivo for 5 days, the quantity of HMG 1 is increased 4-fold in the cytosol, 3.5-fold in the microsomal fraction and 1.6-fold in the nuclear fraction. The finding of large amounts of HMG 1 in cytoplasm of oviduct cell is not likely due to its leakage from the nucleus. We anticipate that HMG 1 is synthesized in the cytoplasm and then transported into the nucleus. The synthesis and transportation of HMG proteins is probably regulated by oestrogen.

已在兔体内诱导出抗鸡输卵管高迁移率族蛋白1（HMG 1）的抗血清。利用这种抗血清，采用免疫生化技术探究雌激素刺激前后鸡输卵管细胞组分中HMG 1的定量变化。在鸡输卵管细胞的胞质溶胶、微粒体和细胞核组分中均可检测到HMG 1。给幼鸡体内注射雌激素5天后，胞质溶胶中HMG 1的量增加了4倍，微粒体组分中增加了3.5倍，细胞核组分中增加了1.6倍。在输卵管细胞质中发现大量HMG 1不太可能是由于其从细胞核泄漏所致。我们推测HMG 1在细胞质中合成，然后转运到细胞核中。HMG蛋白的合成和转运可能受雌激素调节。