钙结合蛋白对膜融合的调节作用。
Modulation of membrane fusion by calcium-binding proteins.
作者信息
Hong K, Düzgüneş N, Papahadjopoulos D
出版信息
Biophys J. 1982 Jan;37(1):297-305. doi: 10.1016/S0006-3495(82)84678-X.
Abstract
The effects of several Ca2+-binding proteins (calmodulin, prothrombin, and synexin) on the kinetics of Ca2+-induced membrane fusion were examined. Membrane fusion was assayed by following the mixing of aqueous contents of phospholipid vesicles. Calmodulin inhibited slightly the fusion of phospholipid vesicles. Bovine prothrombin and its proteolytic fragment 1 had a strong inhibitory effect on fusion. Depending on the phospholipid composition, synexin could either facilitate or inhibit Ca2+-induced fusion of vesicles. The effects of synexin were Ca2+ specific. 10 microM Ca2+ was sufficient to induce fusion of vesicles composed of phosphatidic acid/phosphatidylethanolamine (1:3) in the presence of synexin and 1 mM Mg2+. We propose that synexin may be involved in intracellular membrane fusion events mediated by Ca2+, such as exocytosis, and discuss possible mechanisms facilitating fusion.
摘要
研究了几种钙结合蛋白（钙调蛋白、凝血酶原和突触融合蛋白）对钙离子诱导的膜融合动力学的影响。通过追踪磷脂囊泡中水性内容物的混合来测定膜融合。钙调蛋白对磷脂囊泡的融合有轻微抑制作用。牛凝血酶原及其蛋白水解片段1对融合有强烈抑制作用。根据磷脂组成，突触融合蛋白可以促进或抑制钙离子诱导的囊泡融合。突触融合蛋白的作用具有钙离子特异性。在存在突触融合蛋白和1 mM镁离子的情况下，10 microM钙离子足以诱导由磷脂酸/磷脂酰乙醇胺（1:3）组成的囊泡融合。我们提出突触融合蛋白可能参与由钙离子介导的细胞内膜融合事件，如胞吐作用，并讨论了促进融合的可能机制。
相似文献
1
Modulation of membrane fusion by calcium-binding proteins.
Biophys J. 1982 Jan;37(1):297-305. doi: 10.1016/S0006-3495(82)84678-X.
2
Synexin facilitates fusion of specific phospholipid membranes at divalent cation concentrations found intracellularly.
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4642-4. doi: 10.1073/pnas.79.15.4642.
3
Role of synexin in membrane fusion. Enhancement of calcium-dependent fusion of phospholipid vesicles.
J Biol Chem. 1981 Apr 25;256(8):3641-4.
4
Calcium-dependence of synexin binding may determine aggregation and fusion of lamellar bodies.
Biochem J. 1997 Feb 15;322 ( Pt 1)(Pt 1):103-9. doi: 10.1042/bj3220103.
5
Synexin enhances the aggregation rate but not the fusion rate of liposomes.
Biochemistry. 1988 Jun 14;27(12):4430-9. doi: 10.1021/bi00412a033.
6
Synexin-mediated fusion of bovine chromaffin granule ghosts. Effect of pH.
Biochim Biophys Acta. 1987 Nov 27;905(1):205-12. doi: 10.1016/0005-2736(87)90024-1.
7
In vitro fusion of lung lamellar bodies and plasma membrane is augmented by lung synexin.
Biochim Biophys Acta. 1991 Nov 5;1086(2):157-66. doi: 10.1016/0005-2760(91)90003-z.
8
Synexin-like proteins from human polymorphonuclear leukocytes. Identification and characterization of granule-aggregating and membrane-fusing activities.
J Biol Chem. 1987 Jun 5;262(16):7850-8.
9
Stilbene disulfonic acids inhibit synexin-mediated membrane aggregation and fusion.
Biochim Biophys Acta. 1995 Feb 9;1254(3):274-82. doi: 10.1016/0005-2760(94)00188-5.
10
Effect of synexin on aggregation and fusion of chromaffin granule ghosts at pH 6.
Biochim Biophys Acta. 1987 Oct 2;903(2):309-18. doi: 10.1016/0005-2736(87)90221-5.
引用本文的文献
1
Calcium triggered L alpha-H2 phase transition monitored by combined rapid mixing and time-resolved synchrotron SAXS.
PLoS One. 2008 Apr 30;3(4):e2072. doi: 10.1371/journal.pone.0002072.
2
Fusion of phospholipid vesicles arrested by quick-freezing. The question of lipidic particles as intermediates in membrane fusion.
Biochim Biophys Acta. 1982 Dec 8;693(1):93-8. doi: 10.1016/0005-2736(82)90474-6.
3
Calelectrin self-aggregates and promotes membrane aggregation in the presence of calcium.
EMBO J. 1982;1(10):1167-70. doi: 10.1002/j.1460-2075.1982.tb00008.x.
4
Synexin facilitates fusion of specific phospholipid membranes at divalent cation concentrations found intracellularly.
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4642-4. doi: 10.1073/pnas.79.15.4642.
5
A la recherche du temps perdu--epilogue to the Minkowski Award lecture 1974.
Diabetologia. 1985 Aug;28(8):547-55. doi: 10.1007/BF00281988.
6
Secretagogue and second messenger-activated Cl- permeabilities in isolated pancreatic zymogen granules.
Pflugers Arch. 1989 Oct;415(1):29-36. doi: 10.1007/BF00373138.
7
Ca2+-activated synexin forms highly selective, voltage-gated Ca2+ channels in phosphatidylserine bilayer membranes.
Proc Natl Acad Sci U S A. 1988 May;85(9):2974-8. doi: 10.1073/pnas.85.9.2974.
8
Evaluation of the annexins as potential mediators of membrane fusion in exocytosis.
J Bioenerg Biomembr. 1990 Apr;22(2):97-120. doi: 10.1007/BF00762942.
9
Synexin (annexin VII): a cytosolic calcium-binding protein which promotes membrane fusion and forms calcium channels in artificial bilayer and natural membranes.
J Membr Biol. 1990 Aug;117(2):101-12. doi: 10.1007/BF01868677.
10
Molecular mechanisms of calcium-induced membrane fusion.
J Bioenerg Biomembr. 1990 Apr;22(2):157-79. doi: 10.1007/BF00762944.
本文引用的文献
1
Phosphorus assay in column chromatography.
J Biol Chem. 1959 Mar;234(3):466-8.
2
Movements of labelled calcium in squid giant axons.
J Physiol. 1957 Sep 30;138(2):253-81. doi: 10.1113/jphysiol.1957.sp005850.
3
Studies on the mechanism of membrane fusion: role of phosphate in promoting calcium ion induced fusion of phospholipid vesicles.
Biochemistry. 1980 Dec 23;19(26):6021-9. doi: 10.1021/bi00567a012.
4
Studies on the mechanism of membrane fusion: kinetics of calcium ion induced fusion of phosphatidylserine vesicles followed by a new assay for mixing of aqueous vesicle contents.
Biochemistry. 1980 Dec 23;19(26):6011-21. doi: 10.1021/bi00567a011.
5
Calcium/magnesium specificity in membrane fusion: kinetics of aggregation and fusion of phosphatidylserine vesicles and the role of bilayer curvature.
Biochemistry. 1981 May 26;20(11):3126-33. doi: 10.1021/bi00514a022.
6
Studies on the mechanism of membrane fusion. Role of head-group composition in calcium- and magnesium-induced fusion of mixed phospholipid vesicles.
Biochim Biophys Acta. 1981 Mar 20;642(1):182-95. doi: 10.1016/0005-2736(81)90148-6.
7
Role of synexin in membrane fusion. Enhancement of calcium-dependent fusion of phospholipid vesicles.
J Biol Chem. 1981 Apr 25;256(8):3641-4.
8
Membrane fusion and molecular segregation in phospholipid vesicles.
Biochim Biophys Acta. 1974 May 30;352(1):10-28. doi: 10.1016/0005-2736(74)90175-8.
9
The conversion of prothrombin to thrombin. I. Characterization of the reaction products formed during the activation of bovine prothrombin.
J Biol Chem. 1974 Jan 25;249(2):594-605.
10
Complexes of prothrombin with calcium ions and phospholipids.
J Biol Chem. 1972 May 10;247(9):2747-54.
Zotero 插件