Ruiz P, Scornik J C
J Immunol. 1982 Mar;128(3):1289-95.
The activity of mitogenic factors generated in human mixed lymphocyte cultures (MLC-MF) was analyzed with unstimulated mononuclear leukocytes used as responder cells. Unseparated or adherent, but not nonadherent, irradiated, autologous mononuclear leukocytes were found to significantly enhance the mitogenic activity of MLC-MF. The effect of MLC-MF was not inhibited by hydrocortisone at concentrations that inhibited MLC-MF production, indicating that the MLC-MF effect is direct and is not mediated through further production of mitogenic factors. Experiments in which MLC-MF was produced in a MLC between a HLA-D homozygous individual and her child suggested that soluble alloantigens do not play a role in the MLC-MF effect. Studies in one individual with MLC responses higher than other individuals indicated average MLC-MF production but above average response to MLC-MF. We conclude that MLC-MF is directly mitogenic for unstimulated lymphocytes, without requiring the simultaneously presence of either mitogen or antigen. In addition, the proliferative MLC reaction of antigen. In addition, the proliferative MLC reaction of individuals with strong responses to MLC-MF may be influenced significantly by the nonspecific clones recruited by MLC-MF.
以未受刺激的单核白细胞作为反应细胞，分析了人混合淋巴细胞培养物中产生的促有丝分裂因子（MLC-MF）的活性。发现未分离的、贴壁的而非非贴壁的、经照射的自体单核白细胞能显著增强MLC-MF的促有丝分裂活性。在抑制MLC-MF产生的浓度下，氢化可的松并未抑制MLC-MF的作用，这表明MLC-MF的作用是直接的，并非通过进一步产生促有丝分裂因子介导。在一名HLA-D纯合个体与其孩子之间进行的MLC中产生MLC-MF的实验表明，可溶性同种异体抗原在MLC-MF的作用中不起作用。对一名MLC反应高于其他个体的个体进行的研究表明，其MLC-MF产生量平均，但对MLC-MF的反应高于平均水平。我们得出结论，MLC-MF对未受刺激的淋巴细胞具有直接促有丝分裂作用，无需同时存在促有丝分裂原或抗原。此外，对MLC-MF反应强烈的个体的增殖性MLC反应可能会受到MLC-MF募集的非特异性克隆的显著影响。
