Marsh J C
Cancer Treat Rep. 1982 Mar;66(3):499-504.
The effects of three antifolates, methotrexate (MTX), triazinate (TZT), and a trimethoxyquinazoline derivative (TMQ), and of cytarabine (ara-C) on leukocyte-committed colony-forming cells from three species were compared using an assay allowing in vivo metabolism in an agar diffusion chamber. Dose-survival curves approximated plateaus, and the level of the plateau was variable among species. Canine marrow colony-forming cells were less sensitive to MTX (about 90% survival) than those from mice or man (25%-40% survival). Human marrow colony-forming cells were less sensitive (70% survival) to TZT than those from mice or dogs (25% survival). No interspecies differences were found for TMQ or ara-C. Mouse marrow in situ was less sensitive to iv MTX than mouse marrow in the chamber. No such difference was found for iv TMQ or ara-C. This comparison could not be made for ip TZT.
使用一种可在琼脂扩散室中进行体内代谢的检测方法，比较了三种抗叶酸剂（甲氨蝶呤（MTX）、三嗪酸盐（TZT）和一种三甲氧基喹唑啉衍生物（TMQ））以及阿糖胞苷（ara-C）对三种物种的白细胞定向集落形成细胞的影响。剂量-存活曲线接近平台期，且平台期水平在不同物种间存在差异。犬类骨髓集落形成细胞对MTX的敏感性低于小鼠或人类的骨髓集落形成细胞（犬类约90%存活，小鼠或人类为25%-40%存活）。人类骨髓集落形成细胞对TZT的敏感性低于小鼠或犬类的骨髓集落形成细胞（人类70%存活，小鼠或犬类为25%存活）。未发现TMQ或ara-C存在种间差异。与置于扩散室中的小鼠骨髓相比，原位小鼠骨髓对静脉注射MTX的敏感性较低。静脉注射TMQ或ara-C未发现此类差异。对于腹腔注射TZT无法进行此类比较。
