Burton R S, Feldman M W, Swisher S G
Biochem Genet. 1981 Dec;19(11-12):1237-45. doi: 10.1007/BF00484576.
Linkage relationships among five polymorphic enzyme-coding gene loci in the marine copepod Tigriopus californicus have been determined using electrophoretic analysis of progeny from laboratory matings. Phosphoglucose isomerase (PGI; EC 5.3.1.9) was found to be tightly linked to glutamate-pyruvate transaminase (GPT; EC 2.6..1.2), with only one recombinant observed in 364 progeny; glutamate-oxaloacetate transaminase (GOT; EC 2.6.1.1) is linked to the PGI-GPT pair, with a recombination fraction of approximately 0.20 in male double heterozygotes. Phosphoglucomutase (PGM; EC 2.7.5.1) and an esterase (EST; EC 3.1.1.1) are not linked to the PGI, GPT, GOT grouping, which has been designated linkage group I. Reciprocal crosses have revealed that no recombination occurs in female T. californicus; this observation confirms a previous report that meiosis in female Tigriopus is achiasmatic.
通过对实验室交配产生的后代进行电泳分析，已确定海洋桡足类加州虎斑猛水蚤中五个多态性酶编码基因位点之间的连锁关系。发现磷酸葡萄糖异构酶（PGI；EC 5.3.1.9）与谷氨酸 - 丙酮酸转氨酶（GPT；EC 2.6..1.2）紧密连锁，在364个后代中仅观察到一个重组体；谷氨酸 - 草酰乙酸转氨酶（GOT；EC 2.6.1.1）与PGI - GPT对连锁，在雄性双杂合子中的重组率约为0.20。磷酸葡萄糖变位酶（PGM；EC 2.7.5.1）和一种酯酶（EST；EC 3.1.1.1）与PGI、GPT、GOT分组不连锁，该分组已被指定为连锁群I。正反交实验表明，雌性加州虎斑猛水蚤中不发生重组；这一观察结果证实了先前的一份报告，即雌性虎斑猛水蚤的减数分裂是无交叉的。
