Arluison M, Martres M P, Sokoloff P
C R Seances Soc Biol Fil. 1982;176(1):23-9.
An attempt to localize dopaminergic receptors in the rat neostriatum by high-resolution radioautography was realized using intracerebral injections of the new ligand (antagonist) 3H-domperidone. In tissue regions located far from the injection site, the weaker diffuse radioautographic reaction permitted us to observe the existence of clusters of silver grains over some cerebral structures. The specificity of this type of labelling was tested using intraperitoneal injections of large amounts of haloperidol in order to block the studied receptors. Thus, we observed specific labelling over some nerve terminals as well as a low number of synaptic contact of the asymmetric type (however some synapses of the symmetric type were also labelled). These result agree with our previous work (1), and confirm the existence of dopaminergic synapses in the rat caudate-putamen.
通过脑内注射新配体（拮抗剂）3H-多潘立酮，利用高分辨率放射自显影技术对大鼠新纹状体中的多巴胺能受体进行定位研究。在远离注射部位的组织区域，较弱的弥漫性放射自显影反应使我们能够观察到某些脑结构上银粒簇的存在。为了阻断所研究的受体，通过腹腔注射大量氟哌啶醇来测试这种标记类型的特异性。因此，我们观察到一些神经末梢上有特异性标记，以及少量不对称型突触接触（然而，一些对称型突触也有标记）。这些结果与我们之前的工作（1）一致，并证实大鼠尾状核-壳核中存在多巴胺能突触。
