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[培养的牛淋巴细胞的细胞动力学与放射敏感性]

[Cell kinetics and radiosensitivity of cultured cow lymphocytes].

作者信息

Modave C, Fabry L, Léonard A

出版信息

C R Seances Soc Biol Fil. 1982;176(1):90-4.

PMID:6461401
Abstract

The harlequin-staining technique has been used to study, after PHA-stimulation, the cell proliferation of cow lymphocytes in culture and to assess the radiosensitivity in first mitosis cells. At the 48 h fixation time, only 34% of the cells are in first mitosis whereas 55% are already in second and 11% in third mitosis. The exposure of cow lymphocytes to 200 rad X-rays result in the production of 16% dicentric chromosomes in first mitosis cells.

摘要

采用哈勒quin染色技术研究了PHA刺激后培养的奶牛淋巴细胞的细胞增殖情况,并评估了第一次有丝分裂细胞的放射敏感性。在固定时间为48小时时,只有34%的细胞处于第一次有丝分裂,而55%的细胞已处于第二次有丝分裂,11%的细胞处于第三次有丝分裂。将奶牛淋巴细胞暴露于200拉德的X射线下,导致第一次有丝分裂细胞中产生16%的双着丝粒染色体。

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