Modave C, Fabry L, Léonard A
C R Seances Soc Biol Fil. 1982;176(1):90-4.
The harlequin-staining technique has been used to study, after PHA-stimulation, the cell proliferation of cow lymphocytes in culture and to assess the radiosensitivity in first mitosis cells. At the 48 h fixation time, only 34% of the cells are in first mitosis whereas 55% are already in second and 11% in third mitosis. The exposure of cow lymphocytes to 200 rad X-rays result in the production of 16% dicentric chromosomes in first mitosis cells.
采用哈勒quin染色技术研究了PHA刺激后培养的奶牛淋巴细胞的细胞增殖情况，并评估了第一次有丝分裂细胞的放射敏感性。在固定时间为48小时时，只有34%的细胞处于第一次有丝分裂，而55%的细胞已处于第二次有丝分裂，11%的细胞处于第三次有丝分裂。将奶牛淋巴细胞暴露于200拉德的X射线下，导致第一次有丝分裂细胞中产生16%的双着丝粒染色体。
