Anderson K M, Baranowski J, Economou S G
Ann Clin Lab Sci. 1982 Mar-Apr;12(2):126-33.
Proteins from nine normal rat organs, including heart, lung thymus, liver, spleen, kidney, prostate, abdominal muscle, and brain, were solubilized, separated by electrophoresis according to their different isoelectric points and molecular weights, and stained with Coomassie blue. Patterns of major proteins unique to each organ were identified. Nine "unknown" samples, chosen from the same sources and submitted for analysis in a single blind study, were easily identified by comparing their protein profiles against the nine "reference" patterns. The ability to identify the origin of a tissue sample without recourse to microscopy, by comparing the pattern of its electrophoresed proteins with a "catalogue" of identified protein profiles, provides a prototype for the identification of histologically indeterminate normal and abnormal cells, tissues, and organs. Application of this technique to problems in human pathology and forensic medicine could prove to be very useful.
来自九种正常大鼠器官（包括心脏、肺、胸腺、肝脏、脾脏、肾脏、前列腺、腹肌和大脑）的蛋白质被溶解，根据其不同的等电点和分子量通过电泳进行分离，并用考马斯亮蓝染色。确定了每个器官特有的主要蛋白质模式。从相同来源选取并在单盲研究中提交进行分析的九个“未知”样本，通过将其蛋白质谱与九个“参考”模式进行比较，很容易被识别出来。通过将组织样本的电泳蛋白质模式与已识别蛋白质谱的“目录”进行比较，无需借助显微镜就能识别组织样本来源的能力，为识别组织学上难以确定的正常和异常细胞、组织及器官提供了一个原型。将该技术应用于人类病理学和法医学问题可能会被证明非常有用。
