Novgorodov S A, Dragunova S F, Iaguzhinskiĭ L S
Biofizika. 1982 Mar-Apr;27(2):244-8.
It has been shown that the induction of earlier described system of potassium-dependent transport of hydrogen ions in mitochondria at low pH values of the incubation medium is inhibited by the inhibitors of mitochondria respiratory chain and ATPase. It has been found that antimycin and oligomycin suppress the efflux of potassium ions from mitochondria in the presence of succinic acid. The uncoupler (FCCP) turns the effect of ATPase inhibitors to the efflux of potassium ions and acceleration of mitochondria respiration under experimental conditions. At the same time TMPD removes the effect of antimycin on potassium ion efflux from uncoupled FCCP of mitochondria. The data obtained are explained in terms of the postulate that under experimental conditions along with the system of potassium-dependent ion transport there appears leakage of protons through the ATPase channel. A conclusion is made concerning the control of ion transport induction in mitochondria by the enzymes of oxidative phosphorylation system.
已表明，在孵育介质低pH值条件下，早期描述的线粒体中钾离子依赖性氢离子转运系统的诱导受到线粒体呼吸链抑制剂和ATP酶抑制剂的抑制。已发现，抗霉素和寡霉素在琥珀酸存在下会抑制钾离子从线粒体流出。在实验条件下，解偶联剂（FCCP）会将ATP酶抑制剂的作用转变为钾离子流出并加速线粒体呼吸。同时，TMPD消除了抗霉素对线粒体解偶联FCCP中钾离子流出的影响。根据以下假设对所得数据进行了解释：在实验条件下，除了钾离子依赖性离子转运系统外，质子还会通过ATP酶通道泄漏。得出了关于氧化磷酸化系统的酶对线粒体中离子转运诱导的控制的结论。
