利用巴瑞特显色反应研究突触体肌酸磷酸激酶。
Utilization of Barritt color reaction for studying synaptosomal creatine phosphokinase.
作者信息
Cheng R H, Majlessi M R, Cheng S C
出版信息
Neurochem Res. 1984 Apr;9(4):571-6. doi: 10.1007/BF00964384.
PMID:6462328
Abstract
The assay for creatine phosphokinase activity depending upon the color formation from creatine was reinvestigated. Its sensitivity was improved to 5 nmol of creatine. The method gave satisfactory Michaelis constants for both creatine phosphate and adenosine-diphosphate and was useful in detecting low creatine phosphokinase activity.
摘要
对基于肌酸显色反应的肌酸磷酸激酶活性测定法进行了重新研究。其灵敏度提高到了5纳摩尔肌酸。该方法对磷酸肌酸和二磷酸腺苷都给出了令人满意的米氏常数，可用于检测低水平的肌酸磷酸激酶活性。
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