Graybiel A M, Brecha N, Karten H J
Neuroscience. 1984 May;12(1):191-214. doi: 10.1016/0306-4522(84)90147-7.
The distribution of enkephalin-like immunoreactivity in the superior colliculus has been studied in the cat with the peroxidase-antiperoxidase method. Two striking patterns of immunoreactivity were observed. In the superficial layers there is a thin, dense horizontal band of immunoreactivity in the neuropil of the most dorsal tier of the superficial gray layer (sublamina 1). Because this sublayer corresponds to the zone of densest contralateral retinotectal projection, an intraocular injection of horseradish peroxidase was made in one cat to allow direct comparison of the distributions of opiate-like immunoreactivity and transported tracer in the contralateral superior colliculus. There was a detailed similarity between the two, including the presence of a gap in both at the presumptive site of the optic disc representation. The presence of enkephalin-like immunoreactivity in neural perikarya in and near sublamina 1 of the superficial gray layer, however, raised the possibility that the immunoreactive band is part of an intrinsic opiate system. Deeper in the superficial gray layer there was appreciable but weaker immunoreactivity in the neuropil and fewer immunoreactive neurons. In the intermediate gray layer and, especially medially, even deeper in the superior colliculus, enkephalin-like immunoreactivity was organized into small (100-300 micron wide) patches. In the intermediate gray layer these tended to be arranged periodically, five-seven patches being spaced at 200-600 micron intervals in caudal transverse sections. In some sections adjoining patches appeared to be fused. The patches were absent or difficult to detect in rostral sections. Caudally, they sometimes were adjacent to blood vessels penetrating the intermediate gray layer, but other times were not. Serial section reconstructions suggested that the patches observed in individual sections are part of larger arrays which have the form of anastomotic bands running in longitudinal directions somewhat oblique to the sagittal plane. It is concluded that an opiate mechanism may play a part in controlling the effects of incoming retinal information in the superficial gray layer, directly or indirectly, and that opiate peptides may also act in modulating one or more afferent or efferent systems of the deep collicular layers. Accordingly, from the functional standpoint, enkephalin-like peptides may influence both visual and sensory motor processing in the superior colliculus.
用过氧化物酶 - 抗过氧化物酶法研究了猫上丘中脑啡肽样免疫反应性的分布。观察到两种显著的免疫反应模式。在表层，在浅灰质层最背侧层（亚层1）的神经毡中有一条薄而密集的水平免疫反应带。由于该亚层对应于对侧视网膜 - 顶盖投射最密集的区域，因此在一只猫中进行了眼内注射辣根过氧化物酶，以便直接比较对侧上丘中阿片样免疫反应性和转运示踪剂的分布。两者之间存在详细的相似性，包括在视盘代表的假定部位两者都存在间隙。然而，浅灰质层亚层1及其附近的神经细胞体中存在脑啡肽样免疫反应性，这增加了免疫反应带是内在阿片系统一部分的可能性。在浅灰质层更深的部位，神经毡中有明显但较弱的免疫反应性，免疫反应性神经元较少。在中间灰质层，尤其是在内侧，上丘更深的部位，脑啡肽样免疫反应性组织成小的（100 - 300微米宽）斑块。在中间灰质层，这些斑块倾向于周期性排列，在尾侧横切面上，五到七个斑块以200 - 600微米的间隔分布。在一些相邻的切片中，斑块似乎融合在一起。在 Rostral 切片中斑块不存在或难以检测到。在尾侧，它们有时与穿透中间灰质层的血管相邻，但其他时候则不相邻。连续切片重建表明，在单个切片中观察到的斑块是更大阵列的一部分，这些阵列呈纵向吻合带的形式，与矢状面有些倾斜。结论是，阿片机制可能直接或间接地在控制浅灰质层中传入视网膜信息的效应方面发挥作用，并且阿片肽也可能在调节深层丘状层的一个或多个传入或传出系统中起作用。因此，从功能角度来看，脑啡肽样肽可能影响上丘中的视觉和感觉运动处理。
