Wakabayashi T, Horiuchi M, Kawamoto S, Onda H
Acta Pathol Jpn. 1984 May;34(3):481-8. doi: 10.1111/j.1440-1827.1984.tb07576.x.
Ultrastructure of megamitochondria in mouse hepatocytes induced by cuprizone was analyzed in the light of the fusion of inner mitochondrial membranes. Hepatic parenchymal cells of mice fed with a 0.5% cuprizone-diet for 7-8 days were characterized by megamitochondria with numerous cristae including disoriented, shorter ones than those of the control and those in vesicular configuration. After feeding the animals with cuprizone for 15-16 days, megamitochondria increased their sizes further and the number of cristae decreased drastically. Numbers of cristae based on a unit-mitochondrial area were 41.3 +/- 10.8 for the former type of cristae-enriched megamitochondria and 3.8 +/- 1.8 for the latter type of megamitochondria with the value of 23.9 +/- 6.2 for the control. Considering the content of cytochromes in megamitochondria previously reported, these results might suggest that the fusion and defusion of inner mitochondrial membrane take place along with the fusion of outer membranes during the megamitochondrial formation.
根据线粒体内膜融合情况，对用铜螯合剂诱导的小鼠肝细胞中巨型线粒体的超微结构进行了分析。给小鼠喂食含0.5%铜螯合剂的饲料7 - 8天，其肝实质细胞的特征是巨型线粒体有许多嵴，包括排列紊乱、比对照组短的嵴以及呈泡状结构的嵴。给动物喂食铜螯合剂15 - 16天后，巨型线粒体的尺寸进一步增大，嵴的数量急剧减少。基于单位线粒体面积的嵴数量，前一种富含嵴的巨型线粒体为41.3±10.8，后一种巨型线粒体为3.8±1.8，而对照组为23.9±6.2。考虑到先前报道的巨型线粒体中细胞色素的含量，这些结果可能表明在巨型线粒体形成过程中，线粒体内膜的融合和去融合与外膜的融合同时发生。
