Løvstad R A
Acta Pharmacol Toxicol (Copenh). 1984 May;54(5):340-5. doi: 10.1111/j.1600-0773.1984.tb01940.x.
Copper induced lysis of washed rat erythrocytes was stimulated by catecholamines, the order of effectiveness being: adrenaline greater than noradrenaline approximately equal to dopamine greater than L-DOPA. The degree of effectiveness is related to the rate of copper ion dependent oxidation of catecholamine, adrenaline being more rapidly oxidized than the other catecholamines investigated. Superoxide dismutase, catalase and different hydroxyl radical scavengers (mannitol, tris, formate and ethanol) markedly reduced the haemolytic effect of copper and catecholamine, suggesting the possibility that superoxide radicals and hydrogen peroxide, formed in the reaction system, cooperate in producing hydroxyl radicals, which are directly involved in the haemolytic action. The plasma proteins, ceruloplasmin, albumin and apotransferrin, also reduced the copper-catecholamine induced haemolysis. The protective action is probably not related to the copper binding ability of these proteins.
儿茶酚胺可刺激铜诱导的洗涤大鼠红细胞溶解，其有效性顺序为：肾上腺素＞去甲肾上腺素≈多巴胺＞左旋多巴。有效性程度与儿茶酚胺的铜离子依赖性氧化速率有关，肾上腺素比其他所研究的儿茶酚胺氧化得更快。超氧化物歧化酶、过氧化氢酶和不同的羟自由基清除剂（甘露醇、三羟甲基氨基甲烷、甲酸盐和乙醇）显著降低了铜和儿茶酚胺的溶血作用，这表明反应体系中形成的超氧自由基和过氧化氢可能协同产生直接参与溶血作用的羟自由基。血浆蛋白、铜蓝蛋白、白蛋白和脱铁转铁蛋白也降低了铜 - 儿茶酚胺诱导的溶血。这种保护作用可能与这些蛋白质的铜结合能力无关。
