Suppr超能文献

2-巯基乙醇对正常和慢性铜中毒绵羊肝脏样本中含铜和含锌蛋白质溶解度的影响。

Effects of 2-mercaptoethanol on the solubility of copper and zinc containing proteins in liver samples from normal and chronic copper poisoned sheep.

作者信息

Hussein K S, Frank A, Jones B E, Edqvist L E

出版信息

Acta Vet Scand. 1984;25(1):10-20. doi: 10.1186/BF03547274.

DOI:10.1186/BF03547274
PMID:6464916
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8287455/
Abstract

The solubility of Cu and Zn binding proteins was studied in liver samples from clinically healthy and chronic copper poisoned sheep (CCP). Homogenized liver was divided by ultracentrifugation into cytosol and pellet. The cytosol was gelfiltrated, Cu and Zn contents were determined in the eluates as well as in the pellet. Liver homogenate, cytosol and pellet were treated with 2-mercaptoethanol (ME). The resulting clear solutions were fractionated by gel filtration followed by determination of the contents of the two metals in the eluates. In CCP sheep the solubility of Cu containing proteins from the liver homogenate and pellet increased after incubation with ME. The results suggest that in CCP sheep a considerable amount of Cu containing proteins are present in an insoluble form, which to some extent is resolubilized by ME.

摘要

对临床健康和慢性铜中毒绵羊（CCP）肝脏样本中铜和锌结合蛋白的溶解度进行了研究。将肝脏匀浆通过超速离心分为胞质溶胶和沉淀。对胞质溶胶进行凝胶过滤，测定洗脱液以及沉淀中的铜和锌含量。肝脏匀浆、胞质溶胶和沉淀用2-巯基乙醇（ME）处理。将得到的澄清溶液进行凝胶过滤分级，然后测定洗脱液中两种金属的含量。在CCP绵羊中，肝脏匀浆和沉淀中含铜蛋白与ME孵育后溶解度增加。结果表明，在CCP绵羊中，相当数量的含铜蛋白以不溶性形式存在，ME在一定程度上使其重新溶解。

相似文献

1
Effects of 2-mercaptoethanol on the solubility of copper and zinc containing proteins in liver samples from normal and chronic copper poisoned sheep.2-巯基乙醇对正常和慢性铜中毒绵羊肝脏样本中含铜和含锌蛋白质溶解度的影响。
Acta Vet Scand. 1984;25(1):10-20. doi: 10.1186/BF03547274.
2
Solubility of copper and zinc containing proteins before and after 2-mercaptoethanol treatment of liver samples from normal and from chronically copper poisoned sheep.正常绵羊和长期铜中毒绵羊肝脏样本经2-巯基乙醇处理前后含铜和含锌蛋白质的溶解度
Acta Pharmacol Toxicol (Copenh). 1984 Sep;55(3):247-51. doi: 10.1111/j.1600-0773.1984.tb02045.x.
3
The concentrations of molybdenum and zinc in liver in relation to copper accumulation in normal and copper poisoned sheep.正常及铜中毒绵羊肝脏中钼和锌的浓度与铜蓄积的关系
Acta Vet Scand. 1976;17(3):307-15. doi: 10.1186/BF03547911.
4
Chronic copper poisoning in sheep. ii. The distribution of soluble copper-, molybdenum-, and zinc-binding proteins from liver and kidney.绵羊的慢性铜中毒。ii. 肝脏和肾脏中可溶性铜结合蛋白、钼结合蛋白和锌结合蛋白的分布
Acta Pharmacol Toxicol (Copenh). 1977 Jan;40(1):178-87. doi: 10.1111/j.1600-0773.1977.tb02066.x.
5
Concentration of selenium in liver in relation to copper level in normal and copper-poisoned sheep.正常和铜中毒绵羊肝脏中硒浓度与铜水平的关系
Acta Vet Scand. 1978;19(3):472-4. doi: 10.1186/BF03547619.
6
Chronic copper poisoning in sheep. III. The distribution of soluble copper- and zinc-binding liver proteins of lambs compared with adult sheep.绵羊的慢性铜中毒。III. 与成年绵羊相比，羔羊肝脏中可溶性铜结合蛋白和锌结合蛋白的分布
Acta Pharmacol Toxicol (Copenh). 1977 May;40(5):570-4.
7
Intracellular distribution of copper in the liver of normal and copper loaded sheep.正常及铜负荷绵羊肝脏中铜的细胞内分布
Res Vet Sci. 1979 Jul;27(1):30-7.
8
Copper thionein in the kidneys of copper-poisoned sheep.铜中毒绵羊肾脏中的铜硫蛋白
Chem Biol Interact. 1977 Oct;19(1):13-23. doi: 10.1016/0009-2797(77)90039-4.
9
Copper, zinc and iron levels in the cerebrospinal fluid of copper poisoned sheep.铜中毒绵羊脑脊液中的铜、锌和铁含量
Res Vet Sci. 1979 Nov;27(3):384-5.
10
Copper carrier protein in copper toxic sheep liver.
Res Vet Sci. 1973 Jan;14(1):106-8.

本文引用的文献

1
Metal-dependent properties of metallothionein. Replacement in vitro of zinc in zinc-thionein with copper.金属硫蛋白的金属依赖性特性。在体外将锌硫蛋白中的锌替换为铜。
Biochem J. 1981 Nov 1;199(2):289-95. doi: 10.1042/bj1990289.
2
On the sensitivity of metallothioneins to oxidation during isolation.金属硫蛋白在分离过程中对氧化的敏感性。
Biochem J. 1980 Nov 1;191(2):475-85. doi: 10.1042/bj1910475.
3
Copper toxicosis in sheep.
J Am Vet Med Assoc. 1982 Feb 15;180(4):386-7.
4
Metal binding sites of rat liver Cu-thionein.
Arch Biochem Biophys. 1982 Jan;213(1):109-17. doi: 10.1016/0003-9861(82)90445-3.
5
Studies on the pathogenesis of hepatolenticular degeneration. 3. The effect of copper on rat liver lysosomes.肝豆状核变性发病机制的研究。3. 铜对大鼠肝脏溶酶体的影响。
Am J Pathol. 1968 Dec;53(6):903-27.
6
Neonatal hepatic mitochondrocuprein. 3. Solubilization of the copper and protein from mitochondria of newborn liver by reduction with mercaptoethanol.新生儿肝脏线粒体铜蛋白。3. 用巯基乙醇还原从新生肝脏线粒体中溶解铜和蛋白质。
Biochim Biophys Acta. 1968 Jan 22;154(1):236-8. doi: 10.1016/0005-2795(68)90280-8.
7
Chronic copper toxicity of ruminants.反刍动物的慢性铜中毒
Proc Nutr Soc. 1969 Sep;28(2):189-98. doi: 10.1079/pns19690037.
8
Hepatic copper-and zinc-binding proteins in ruminants. 1. Distribution of Cu and Zn among soluble proteins of livers of varying Cu and Zn content.
Br J Nutr. 1974 Sep;32(2):283-91. doi: 10.1079/bjn19740081.
9
Intracellular distribution of hepatic copper in normal and copper-loaded sheep.正常和铜负荷绵羊肝脏铜的细胞内分布
J Anim Sci. 1978 Nov;47(5):1174-9. doi: 10.2527/jas1978.4751174x.
10
Chronic copper poisoning in sheep. I. The relationship of methaemoglobinemia to Heinz body formation and haemolysis during the terminal crisis.绵羊的慢性铜中毒。I. 终末期危象期间高铁血红蛋白血症与海因茨小体形成及溶血的关系。
Acta Pharmacol Toxicol (Copenh). 1977 Jan;40(1):169-77.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验