2-巯基乙醇对正常和慢性铜中毒绵羊肝脏样本中含铜和含锌蛋白质溶解度的影响。

Effects of 2-mercaptoethanol on the solubility of copper and zinc containing proteins in liver samples from normal and chronic copper poisoned sheep.

作者信息

Hussein K S, Frank A, Jones B E, Edqvist L E

出版信息

Acta Vet Scand. 1984;25(1):10-20. doi: 10.1186/BF03547274.

DOI:10.1186/BF03547274

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8287455/

Abstract

The solubility of Cu and Zn binding proteins was studied in liver samples from clinically healthy and chronic copper poisoned sheep (CCP). Homogenized liver was divided by ultracentrifugation into cytosol and pellet. The cytosol was gelfiltrated, Cu and Zn contents were determined in the eluates as well as in the pellet. Liver homogenate, cytosol and pellet were treated with 2-mercaptoethanol (ME). The resulting clear solutions were fractionated by gel filtration followed by determination of the contents of the two metals in the eluates. In CCP sheep the solubility of Cu containing proteins from the liver homogenate and pellet increased after incubation with ME. The results suggest that in CCP sheep a considerable amount of Cu containing proteins are present in an insoluble form, which to some extent is resolubilized by ME.

摘要

对临床健康和慢性铜中毒绵羊（CCP）肝脏样本中铜和锌结合蛋白的溶解度进行了研究。将肝脏匀浆通过超速离心分为胞质溶胶和沉淀。对胞质溶胶进行凝胶过滤，测定洗脱液以及沉淀中的铜和锌含量。肝脏匀浆、胞质溶胶和沉淀用2-巯基乙醇（ME）处理。将得到的澄清溶液进行凝胶过滤分级，然后测定洗脱液中两种金属的含量。在CCP绵羊中，肝脏匀浆和沉淀中含铜蛋白与ME孵育后溶解度增加。结果表明，在CCP绵羊中，相当数量的含铜蛋白以不溶性形式存在，ME在一定程度上使其重新溶解。

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