Küng W, Fabbro D, Handloser K, Eppenberger U
Anticancer Res. 1984 May-Jun;4(3):145-50.
The soluble cAMP-dependent protein kinase activities of two estrogen receptor-containing (MCF-7 and ZR-75-1) and two estrogen receptor-lacking (BT-20 and MDA-MB-231) established human mammary tumor cell lines were analyzed by DEAE-cellulose chromatography and photoaffinity labeling with 8-azido-[32P]cAMP. Predominantly, type I isoenzyme was present in MDA-MB-231 cells, type II protein kinase was the main form in ZR-75-1 and BT-20 cells; whereas MCF-7 cytosols contained equal amounts of both protein kinase types. No correlations between estrogen receptor content and cAMP-dependent protein kinase holoenzyme ratios of isoenzymes were found. A distinctly greater heterogeneity of charge isomers of cAMP-binding proteins (regulatory subunits) was observed in estrogen receptor-containing cells.
通过DEAE - 纤维素色谱法和用8 - 叠氮基 - [³²P]cAMP进行光亲和标记，分析了两种含雌激素受体的（MCF - 7和ZR - 75 - 1）以及两种缺乏雌激素受体的（BT - 20和MDA - MB - 231）已建立的人乳腺肿瘤细胞系的可溶性环磷酸腺苷（cAMP）依赖性蛋白激酶活性。主要地，I型同工酶存在于MDA - MB - 231细胞中，II型蛋白激酶是ZR - 75 - 1和BT - 20细胞中的主要形式；而MCF - 7细胞溶质中两种蛋白激酶类型的含量相等。未发现雌激素受体含量与同工酶的cAMP依赖性蛋白激酶全酶比率之间存在相关性。在含雌激素受体的细胞中观察到环磷酸腺苷结合蛋白（调节亚基）的电荷异构体有明显更大的异质性。
