单价阳离子（Na⁺、K⁺）和二价阳离子（Ca²⁺、Mg²⁺）对乙酰胆碱酯酶的激活作用。
Activation of acetylcholinesterase by monovalent (Na+,K+) and divalent (Ca2+,Mg2+) cations.
作者信息
Hofer P, Fringeli U P, Hopff W H
出版信息
Biochemistry. 1984 Jun 5;23(12):2730-4. doi: 10.1021/bi00307a030.
The activation of acetylcholinesterase [EC 3.1.1.7 (AChE)] by monovalent and divalent metal ions has been investigated by kinetic experiments under steady-state conditions (pH-stat method). It has been shown that at low ionic strength the enhancement of the activity by both monovalent and divalent metal ions can be explained as an electrostatic effect. Thereby, enhancement of the concentration of monovalent metal ions (Na+ and K+) acts by reducing the penetration depth of the electric field of carboxylate groups located at the active center whereby divalent metal ions (Ca2+ and Mg2+) act by a complex formation with these charged groups.
在稳态条件下（pH 计法）通过动力学实验研究了单价和二价金属离子对乙酰胆碱酯酶[EC 3.1.1.7（AChE）]的激活作用。结果表明，在低离子强度下，单价和二价金属离子对活性的增强可解释为静电效应。因此，单价金属离子（Na⁺和 K⁺）浓度的增加是通过降低位于活性中心的羧酸根基团电场的穿透深度来实现的，而二价金属离子（Ca²⁺和 Mg²⁺）则是通过与这些带电基团形成络合物来发挥作用的。
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