[尿素水溶液中的DNA构象与热稳定性]
[DNA conformation and thermostability in urea aqueous solutions].
作者信息
Aslanian V M, Babaian Iu S, Arutiunian S G
出版信息
Biofizika. 1984 May-Jun;29(3):372-6.
It is suggested from the character of the change of circular dichroism spectra that in the presence of urea winding of DNA double helix takes place within the bounds of B-family of forms. It is shown that the realized conformation of DNA differs from the experimentally known forms of DNA belonging to B-family. Urea destabilizes the DNA molecules without connection with helical and melt pairs of DNA nitrous bases. Urea affects the conformational state of DNA by water destruction around DNA, which is accompanied by dehydration of DNA and basic metal ions.
从圆二色光谱变化的特征表明，在尿素存在的情况下，DNA双螺旋的缠绕发生在B型家族形式的范围内。结果表明，DNA实现的构象不同于实验已知的属于B型家族的DNA形式。尿素使DNA分子不稳定，与DNA亚硝酸盐碱基的螺旋和熔解对无关。尿素通过破坏DNA周围的水来影响DNA的构象状态，这伴随着DNA和碱性金属离子的脱水。
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